Metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species

Abstract

The present invention relates to a metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species. The metal oxide nano-composite magnetic material comprises the magnetic iron oxide nanoparticle, a silica layer immobilized onto the magnetic iron oxide nanoparticle and a metal oxide layer coated onto the silica layer. The magnetic iron oxide nanoparticles can be used for absorbing microwave radiation to accelerate the enrichment and linkage for phosphorylated species onto the metal oxide nano-composite magnetic material. Furthermore, the magnetic property of magnetic iron oxide nanoparticles leads to isolation of the metal oxide nano-composite magnetic material-target species conjugates by simply employing an external magnetic field. The specificity of the metal oxide on the surface of the metal oxide nano-composite magnetic material for phosphorylated species also leads to the linkage/enrichment of phosphorylated species with high selectivity.

1 . Method for linkage, enrichment, and isolation of phosphorylated species on the metal oxide nano-composite magnetic material comprises the following steps:
 mixing metal oxide nano-composite magnetic material with sample solution containing phosphorylated species;   carrying out linkage and enrichment of phosphorylated species on the metal oxide nano-composite magnetic material under either microwave heating or pipetting; and   isolating the metal oxide nano-composite magnetic material-phosphorylated species conjugates from the sample solution by employing an external magnet.   
     
     
         2 . Method for linkage, enrichment, and isolation of phosphorylated species on the metal oxide nano-composite magnetic material of  claim 1 , wherein the step of carrying out linkage and enrichment of phosphorylated species on the metal oxide nano-composite magnetic material under microwave heating for 30˜90 seconds. 
     
     
         3 . Method for linkage, enrichment, and isolation of phosphorylated species on the metal oxide nano-composite magnetic material of  claim 1 , further comprising a step of confirming the phosphorylated species enriched by the metal oxide nano-composite magnetic material is carried out by MALDI MS after the step of isolating the nano-composite magnetic material-phosphorylated species conjugates from the sample solution by employing an external magnet. 
     
     
         4 . Method for linkage, enrichment, and isolation of phosphorylated species on the metal oxide nano-composite magnetic material of  claim 1 , wherein the step of mixing metal oxide nano-composite magnetic material with sample solution containing phosphorylated species is carried out in aqueous trifluoroacetic acid. 
     
     
         5 . Method for linkage, enrichment, and isolation of phosphorylated species on the metal oxide nano-composite magnetic material of  claim 1 , wherein the step of carrying out linkage and enrichment of phosphorylated species on the metal oxide nano-composite magnetic material under either microwave heating or pipetting is based on the chelating capability of phosphates on the phosphorylated species and the metal on metal oxides such as aluminum and niobium on the metal oxide nano-composite magnetic material.