Reduced risk tobacco products and use thereof

Abstract

Reduced risk tobacco-related products and methods of use. These tobacco-related products (e.g., cigarettes or filters) are designed to reduce the biological insult, for example, DSBs, cell death or perturbation of RNA transcriptome or proteome as compared to the amount of biological insult induced by another cigarette, such as a conventional or reference cigarette (e.g., 2R4F), in human cells, and to provide a reduced risk cigarette that meets a cigarette smoker's sensory/perception needs.

1 . A cigarette comprising:
 a blend of cured tobacco comprising a Burley tobacco and a Flue-cured tobacco; and   a filter comprising at least 70 mg of carbon and at least 30 mg of resin, wherein the cigarette is configured to produce a mainstream smoke that generates fewer DNA double strand breaks (DNA DSBs) in human cells than the mainstream smoke from a reference cigarette under the same smoking conditions.   
     
     
         2 . The cigarette of  claim 1 , wherein the Burley tobacco is present in an amount of about 45-70% by weight based on the combined weight of the blend, and the Flue-cured tobacco is present in an amount of about 55-30% by weight based on the combined weight of the blend. 
     
     
         3 . The cigarette of  claim 1 , wherein the resin is a weak base amine-containing resin. 
     
     
         4 . The cigarette of  claim 1 , wherein said filter comprises at least 100 mg of carbon and at least 30 mg of resin. 
     
     
         5 . The cigarette of  claim 2 , wherein said filter comprises at least 100 mg of carbon and at least 30 mg of resin. 
     
     
         6 . The cigarette of  claim 4 , wherein the resin is a weak base amine-containing resin. 
     
     
         7 . The cigarette of  claim 5 , wherein the resin is a weak base amine-containing resin. 
     
     
         8 . The cigarette of  claim 1 , wherein the reference cigarette is IM16, 2R4F, or 1R5F. 
     
     
         9 . The cigarette of  claim 2 , wherein the reference cigarette is IM16, 2R4F, or 1R5F. 
     
     
         10 . The cigarette of  claim 1 , wherein the human cells are human lung cells. 
     
     
         11 . The cigarette of  claim 2 , wherein the human cells are human lung cells. 
     
     
         12 . A cigarette comprising:
 a blend of cured tobacco comprising a Burley tobacco and a Flue-cured tobacco; and   a filter comprising at least 100 mg of carbon, wherein the cigarette is configured to produce a mainstream smoke that generates fewer DNA double strand breaks (DNA DSBs) in human cells than the mainstream smoke from a reference cigarette under the same smoking conditions.   
     
     
         13 . The cigarette of  claim 12 , wherein the Burley tobacco is present in an amount of about 45-70% by weight based on the combined weight of the blend, and the Flue-cured tobacco is present in an amount of about 55-30% by weight based on the combined weight of the blend. 
     
     
         14 . The cigarette of  claim 12 , wherein the filter further comprises a weak base amine-containing resin. 
     
     
         15 . The cigarette of  claim 13 , wherein the filter further comprises a weak base amine-containing resin. 
     
     
         16 . The cigarette of  claim 12 , wherein the reference cigarette is IM16, 2R4F, or 1R5F. 
     
     
         17 . The cigarette of  claim 13 , wherein the reference cigarette is IM16, 2R4F, or 1R5F. 
     
     
         18 . The cigarette of  claim 12 , wherein the human cells are human lung cells. 
     
     
         19 . The cigarette of  claim 13 , wherein the human cells are human lung cells. 
     
     
         20 . A cigarette filter comprising at least 70 mg of carbon and at least 30 mg of resin, wherein the filter is configured to reduce DNA double strand breaks (DNA DSBs) in mainstream smoke. 
     
     
         21 . The cigarette filter of  claim 20 , wherein the resin is a weak base amine-containing resin. 
     
     
         22 . The cigarette filter of  claim 20 , wherein the filter comprises at least 100 mg of carbon and at least 30 mg of resin. 
     
     
         23 . The cigarette filter of  claim 20 , wherein the resin is a weak base amine-containing resin. 
     
     
         24 . A cigarette filter comprising at least 100 mg of carbon, wherein the filter is configured to reduce DNA double strand breaks (DNA DSBs) in mainstream smoke. 
     
     
         25 . The cigarette filter of  claim 24 , further comprising a weak base amine-containing resin. 
     
     
         26 . A method of making a filtered cigarette comprising:
 preparing a blend of cured tobacco comprising a Burley tobacco and a Flue-cured tobacco;   incorporating at least 70 mg of carbon and at least 30 mg of resin into a cigarette filter; and   generating a filtered cigarette that contains said blend of cured tobacco and said cigarette filter, wherein the cigarette is configured to produce a mainstream smoke that generates fewer DNA double strand breaks (DNA DSBs) in lung cells than the mainstream smoke from a reference cigarette under the same smoking conditions.   
     
     
         27 . The method of  claim 26 , wherein the Burley tobacco is present in an amount of about 45-70% by weight based on the combined weight of the blend, and the Flue-cured tobacco is present in an amount of about 55-30% by weight based on the combined weight of the blend. 
     
     
         28 . The method of  claim 26 , wherein said filter comprises at least 100 mg of carbon and at least 30 mg of resin. 
     
     
         29 . A method of making a filtered cigarette comprising:
 preparing a blend of cured tobacco comprising a Burley tobacco and a Flue-cured tobacco;   incorporating at least 100 mg of carbon into a cigarette filter; and   generating a filtered cigarette that contains said blend of cured tobacco and said cigarette filter, wherein the cigarette is configured to produce a mainstream smoke that generates fewer DNA double strand breaks (DNA DSBs) in lung cells than the mainstream smoke from a reference cigarette under the same smoking conditions.   
     
     
         30 . The method of  claim 29 , wherein the Burley tobacco is present in an amount of about 45-70% by weight based on the combined weight of the blend, and the Flue-cured tobacco is present in an amount of about 55-30% by weight based on the combined weight of the blend.