US2010209406A1PendingUtilityA1
Bacillus strains and methods for reducing e. coli disease and enhancing performance
Est. expiryMay 14, 2024(expired)· nominal 20-yr term from priority
A61P 3/00A23K 10/18A23K 50/30A61K 35/742C12R 2001/07C12N 1/205
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Abstract
Bacillus strains that inhibit pathogenic swine E. coli and/or improve performance are provided. Inhibition of pathogenic swine E. coli decreases E. coli disease. At least one strain enhanced swine performance by improving average daily gain, feed efficiency, and feed intake. Preferred Bacillus strains are of species that are included on the GRAS list. Bacillus species are sporeformers and therefore are highly stable and can be fed to swine.
Claims
exact text as granted — not AI-modified1 . An isolated microorganism having all of the characteristics of Bacillus strain 3A-P4 ATCC PTA-6506.
2 . An isolated microorganism having all of the characteristics of Bacillus strain 15A-P4 ATCC PTA-6507.
3 . An isolated microorganism having all of the characteristics of Bacillus strain 22C-P1 ATCC PTA-6508.
4 . A method of feeding one or more animal, the method comprising feeding to the one or more animal an isolated microorganism of the genus Bacillus selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508 and mixtures thereof.
5 . The method of claim 4 , wherein the microorganism is strain 15A-P4 ATCC PTA-6507.
6 . The method of claim 4 , wherein the mixtures thereof comprise at least two strains.
7 . The method of claim 6 , wherein the strains comprise strain 22C-P1 ATCC PTA-6508 and strain 15A-P4 ATCC PTA-6507.
8 . The method of claim 7 , wherein the microorganism comprise 90% of the total count of strain 22C-P1 ATCC PTA-6508 and 10% of the total count of strain 15A-P4 ATCC PTA-6507.
9 . The method of claim 7 , wherein the microorganism comprise 10% of the total count of strain 22C-P1 ATCC PTA-6508 and 90% of the total count of strain 15A-P4 ATCC PTA-6507.
10 . A method of feeding one or more animal, the method comprising feeding to the one or more animal a combination of microorganisms of the genus Bacillus , the combination comprising strains 22C-P1 ATCC PTA-6508 and 15A-P4 ATCC PTA-6507.
11 . A method of feeding one or more fowl, the method comprising feeding to the one or more fowl an isolated microorganism of the genus Bacillus selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508 and mixtures thereof.
12 . The method of claim 11 , wherein the microorganism is strain 15A-P4 ATCC PTA-6507.
13 . The method of claim 11 , wherein the mixtures thereof comprise at least two strains.
14 . The method of claim 13 , wherein the strains comprise strain 22C-P1 ATCC PTA-6508 and strain 15A-P4 ATCC PTA-6507.
15 . The method of claim 14 , wherein the strains comprise 90% of the total count of strain 22C-P1 ATCC PTA-6508 and 10% of the total count of strain 15A-P4 ATCC PTA-6507.
16 . The method of claim 14 , wherein the strains comprise 10% of the total count of strain 22C-P1 ATCC PTA-6508 and 90% of the total count of strain 15A-P4 ATCC PTA-6507.
17 . A method of feeding one or more fowl, the method comprising feeding to the one or more fowl a combination comprising Bacillus strains 22C-P1 ATCC PTA-6508 and 15A-P4 ATCC PTA-6507.
18 . A method of feeding a pig, the method comprising feeding to the pig an isolated microorganism of the genus Bacillus selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508 and mixtures thereof.
19 . A method of feeding swine, the method comprising feeding to the swine one or more isolated microorganism of the genus Bacillus selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508 and mixtures thereof.
20 . A method of forming a direct-fed microbial, the method comprising:
(a) growing, in a liquid nutrient broth, a Bacillus strain selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508; and (b) separating the strain from the liquid to form the direct-fed microbial.
21 . A method of feeding swine, the method comprising feeding to the swine an isolated microorganism of the genus Bacillus selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508 and mixtures thereof.
22 . A method of forming a direct-fed microbial, the method comprising:
(a) growing, in a liquid nutrient broth, a Bacillus strain 22C-P1 ATCC PTA-6508; and (b) separating the strain from the liquid to form the direct-fed microbial.Cited by (0)
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