Aqueous transfer buffer

Abstract

The present invention relates to an aqueous transfer buffer that provides superior efficiency in transferring polypeptides of a broad range of molecular weight from a matrix used in electrophoresis to another immobilized surface. Also disclosed are electrophoretic methods and devices in which the aqueous transfer solution of this invention is used.

1 . An aqueous solution comprising Tris at a concentration of at least 300 mM and glycine at a concentration of at least 300 mM. 
     
     
         2 . The solution of  claim 1 , further comprising ethanol or methanol at a concentration of no greater than 20% by weight. 
     
     
         3 . The solution of  claim 1 , further comprising a detergent at a concentration of no greater than 0.1% by weight. 
     
     
         4 . The solution of  claim 3 , wherein the detergent is SDS. 
     
     
         5 . The solution of  claim 1 , wherein the concentration of Tris is 300 mM. 
     
     
         6 . The solution of  claim 1 , wherein the concentration of glycine is 300 mM. 
     
     
         7 . The solution of  claim 1 , which has a pH of about 9.0. 
     
     
         8 . The solution of  claim 1 , wherein the concentration of Tris is 300 mM, the concentration of glycine is 300 mM, and the solution has a pH of about 9.0. 
     
     
         9 . The solution of  claim 1 , wherein the concentration of Tris is 400 mM, the concentration of glycine is 400 mM, and the solution has a pH of about 9.0. 
     
     
         10 . The solution of  claim 1 , wherein the concentration of Tris is 500 mM, the concentration of glycine is 500 mM, and the solution has a pH of about 9.0. 
     
     
         11 . The solution of  claim 1 , wherein the concentration of Tris or glycine is 1 M or higher. 
     
     
         12 . The solution of  claim 11 , wherein the concentration of Tris is 1 M, the concentration of glycine is 1 M, and the solution has a pH of about 9.0. 
     
     
         13 . The solution of  claim 11 , wherein the concentration of Tris is 250 mM, the concentration of glycine is 1.92 M, and the solution has a pH of about 8.3.