Mhc-peptide complex binding ligands

Abstract

Disclosed are protein ligands comprising an immunoglobulin heavy chain variable (VH) domain and an immunoglobulin light chain variable (VL) domain, wherein the proteins bind a complex comprising an MHC and a peptide, do not substantially bind the MHC in the absence of the bound peptide, and do not substantially bind the peptide in the absence of the MHC, and the peptide is a peptide fragment of gp100, MUC1, TAX, or hTERT. Also disclosed are methods of using and identifying such ligands.

1 - 3 . (canceled) 
     
     
         4 . A protein comprising an immunoglobulin heavy chain variable (VH) domain and an immunoglobulin light chain variable (VL) domain, wherein the protein binds a complex comprising an MHC and a peptide, does not substantially bind said MHC in an absence of said peptide, and does not substantially bind said peptide in an absence of said MHC, wherein said peptide is a peptide fragment of gp100, MUC1 or TAX. 
     
     
         5 . The protein of  claim 4 , wherein said MHC is MHC class I. 
     
     
         6 . The protein of  claim 5 , wherein said MHC class I comprises HLA-A*0201. 
     
     
         7 . The protein of  claim 4 , wherein said peptide fragment of said gp100 is G9-209 (SEQ ID NO:1). 
     
     
         8 . The protein of  claim 4 , wherein said peptide fragment of said gp100 is G9-280 (SEQ ID NO:2). 
     
     
         9 . The protein of  claim 4 , wherein said peptide fragment of said gp100 is G9-154 (SEQ ID NO:3). 
     
     
         10 . The protein of  claim 4 , wherein said peptide fragment of said MUC1 is D6 (SEQ ID NO:4). 
     
     
         11 . The protein of  claim 4 , wherein said peptide fragment of said TAX is set forth by SEQ ID NO:121. 
     
     
         12 . The protein of  claim 7 , wherein said protein comprises all six CDRs of 1A11 (SEQ ID NOs:8 and 10), 1A7 (SEQ ID NOs:12 and 14), 1A9 (SEQ ID NOs:16 and 18), 1C8 (SEQ ID NOs:20 and 22), 1D7 (SEQ ID NOs:24 and 26) or 1G2 (SEQ ID NOs:28 and 30). 
     
     
         13 . The protein of  claim 8 , wherein said protein comprises all six CDRs of 2B2 (SEQ ID NOs:32 and 34), 2C5 (SEQ ID NOs:36 and 38), 2D1 (SEQ ID NOs:40 and 42) or 2F1 (SEQ ID NOs:44 and 46). 
     
     
         14 . The protein of  claim 9 , wherein said protein comprises all six CDRs of G2D12 (SEQ ID NOs:48 and 50), G3F12 (SEQ ID NOs:52 and 54), G3F3 (SEQ ID NOs:56 and 58) or G3G4 (SEQ ID NOs:60 and 62). 
     
     
         15 . The protein of  claim 10 , wherein said protein comprises all six CDRs of M3A1 (SEQ ID NOs:64 and 66) or M3B8 (SEQ ID NOs:68 and 70). 
     
     
         16 . The protein of  claim 10 , wherein said protein comprises all six CDRs of T3E3 (SEQ ID NOs:72 and 74), T3F1 (SEQ ID NOs:76 and 78) or T3F2 (SEQ ID NOs:80 and 82).