Method for purifying cancer-specific proliferating cell nuclear antigen

Abstract

An isolated antibody that binds a cancer specific Proliferating Cell Nuclear Antigen (csPCNA) that binds to the amino acid sequence LeuLysGlnLeuAspAlaGlnGlnThrGlnLeuArgIle AspSerPhePheArgLeuAlaGlnGlnGluLysGluAspAlaLysArg (SEQ ID No. 1). An immunoassay that utilizes an isolated antibody that binds a form of csPCNA that binds to the SEQ ID No. 1 to identify the presence of csPCNA in a sample.

1 . An antibody that binds to a cancer specific Proliferating Cell Nuclear Antigen (csPCNA), wherein said csPCNA binds to the amino acid sequence corresponding to SEQ ID. No. 1. 
     
     
         2 . The antibody of  claim 1 , wherein the antibody is a monoclonal antibody. 
     
     
         3 . The antibody of  claim 1 , wherein the antibody is a polyclonal antibody. 
     
     
         4 . The antibody of  claim 1 , wherein the antibody is a recombinant antibody. 
     
     
         5 . The antibody of  claim 1 , wherein said antibody is used in an immunoassay. 
     
     
         6 . A method for isolating an antibody that binds to a csPCNA, comprising the steps of:
 (A) obtaining a sample,   (B) contacting said sample with a peptide comprising SEQ ID No. 1, forming a csPCNA-peptide complex,   (C) isolating said csPCNA from said csPCNA-peptide complex,   (D) producing an antibody that binds to csPCNA, wherein said csPCNA binds to the aminoacid sequence corresponding to SEQ ID No. 1, and   (E) isolating said antibody.   
     
     
         7 . The method of  claim 6 , wherein said antibody is produced by challenging a mammal with the isolated csPCNA of step C. 
     
     
         8 . The method of  claim 7 , wherein said mammal is selected from the group consisting of rabbits, goats, horses, mice, rats, and sheep. 
     
     
         9 . The method of  claim 6 , wherein said antibody is produced by challenging a hen with said isolated csPCNA.