Compiled Methods for Analysing and Sorting Samples

Abstract

The present invention relates to the fields of analysis, diagnostics, prognostics, standardization, characterization and enumeration of entities such as biological cells, as well as therapeutical applications. Accordingly, the present invention in preferred aspects is directed to methods for the detection and/or analysis and/or isolation and optionally further manipulation of entities, such as cells, particles, and supra-molecular structures.

1 . A method for detecting and/or analysing and/or partitioning one or more entities present in a sample, said method comprising the steps of
 A) providing a sample by extracting the sample from a sample source,   B) preparing the sample for assaying by contacting said sample with one or more of i) a marker molecule specific for the one or more entities; ii) a labelling molecule specific for the one or more entities; and iii) a detection molecule comprising a marker molecule and a labelling molecule specific for the one or more entities, and   C) assaying the sample at least for the presence of the one or more entities, and optionally one or more further steps selected from   D) data processing and/or sample processing, such as sample analysis and/or partitioning of analysed entities, and   E) data interpretation and/or   F) further sample manipulations, such as cultivation and/or expansion of partitioned and/or isolated cells.   
     
     
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