new type of consensus interferon and preparation method thereof

Abstract

The invention relates to variants of consensus interferon protein with improved properties, such as improved anti-viral activity, and use thereof. The variants are also easier to renature after denaturant treatment. The invention also relates to the preparation method of the variants consensus interferon.

1 . A variant consensus interferon protein comprising the amino acid sequence of SEQ ID NO: 3. 
     
     
         2 . The variant consensus interferon protein of  claim 1 , further comprising a modification at position S156, wherein said modification is S156C. 
     
     
         3 . The variant consensus interferon protein of  claim 2 , further comprising at least one polyethylene glycol (PEG) moiety covalently attached to the protein. 
     
     
         4 . The variant consensus interferon protein of  claim 3 , wherein said (PEG) moiety is attached at the S156C position of the protein. 
     
     
         5 . The variant consensus interferon protein of  claim 3  or  4 , wherein said PEG moiety is (PEG)n. 
     
     
         6 . The variant consensus interferon protein of any one of  claims 2 - 5 , wherein said PEG moiety is a PEG derivative. 
     
     
         7 . A DNA encoding the consensus interferon variant of  claim 1  or  2 . 
     
     
         8 . An expression vector comprising the DNA of  claim 7 . 
     
     
         9 . A host cell comprising the DNA of  claim 7 . 
     
     
         10 . A host cell transformed with the expression vector of  claim 8 . 
     
     
         11 . The host cell of  claim 9  or  10 , wherein said host cell is a prokaryote cell. 
     
     
         12 . The host cell of  claim 11 , wherein said host cell is  E. coli.    
     
     
         13 . A pharmaceutical composition comprising the variant consensus interferon protein according to any one of  claim 1  and a pharmaceutically acceptable excipient. 
     
     
         14 . A method of manufacturing a consensus interferon, the method comprising the steps of:
 growing the host cell of  claim 9  under a condition that the interferon consensus variants can be expressed;   harvesting the host cell; and   purifying the interferon consensus variants.   
     
     
         15 . A method of suppressing viral activity in a subject, the method comprising administering an animal or human subject with the variant consensus interferon protein according to any one of  claim 1 .