Antibody having inhibitory effect on amyloid fibril formation

Abstract

Disclosed is an antibody having a high inhibitory effect on amyloid fibril formation. An antibody is produced by using a liposome containing a GM1 ganglioside at a predetermined ratio as an immunogen. Thus, the sequences of four types of antibodies each having a high inhibitory effect on amyloid fibril formation can be provided.

1 : An isolated antibody comprising:
 a combination of a heavy chain variable region comprising a heavy chain CDR1 having the amino acid sequence of SEQ ID NO: 26, a heavy chain CDR2 having the amino acid sequence of SEQ ID NO: 27, and a heavy chain CDR3 having the amino acid sequence of SEQ ID NO: 28, and   a light chain variable region comprising a light chain CDR1 having the amino acid sequence of SEQ ID NO: 30, a light chain CDR2 having the amino acid sequence of SEQ ID NO: 31, and a light chain CDR3 having the amino acid sequence of SEQ ID NO: 32; and   wherein the antibody recognizes GM1 ganglioside-bound amyloid β-protein.   
     
     
         2 : The isolated antibody according to  claim 1 , wherein the heavy chain variable region has the amino acid sequence of SEQ ID NO: 25 and
 the light chain variable region has the amino acid sequence of SEQ ID NO: 29.   
     
     
         3 : The isolated antibody according to  claim 1 , wherein the antibody is a humanized antibody. 
     
     
         4 : The isolated antibody according to  claim 1 , wherein the antibody is Fab, Fab′, F(ab′) 2 , scFv, or dsFv antibody. 
     
     
         5 : The isolated antibody according to  claim 1 , wherein the antibody has an inhibitory effecting in an amyloid β protein polymerization inhibition test in vitro of 50% or more.