Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium

Abstract

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof. The cell line, the lysate, the extract, and the culture medium has minimized side effects compared to the conventional therapeutic drugs, is safe to the human body, is involved directly in the growth of cancer to induce cancer cell death, and shows anticancer activity of inhibiting angiogenesis occurring in carcinogenesis. Accordingly, the cell line, the lysate, the extract and the culture medium is useful for the prevention, treatment and alleviation of cancer.

1 . A pharmaceutical composition for preventing or treating cancer, which contains any one or more selected from the group consisting of a cell line, which is derived from the cambium or procambium of  Taxus  and has the following characteristics; a lysate thereof; an extract thereof; and a culture medium thereof:
 (a) it is morphologically characterized by a large number of vacuoles;   (b) it is in an innately undifferentiated state; and   (c) it is a homogeneous cell line.   
     
     
         2 . The pharmaceutical composition for preventing or treating cancer according to  claim 1 , wherein the cell line is additionally characterized in that: (a) it is present as single cells during suspension culture; (b) it has a growth rate higher than those of cell lines derived from tissues other than the cambium or procambium of  Taxus  and is cultured stably; and (c) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium or procambium of  Taxus.    
     
     
         3 . The pharmaceutical composition for preventing or treating cancer according to  claim 1 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a  Taxus  cambium- or procambium-containing tissue;   (b) inducing a cambium or procambium layer proliferated from the cambium or procambium and an amorphous callus layer proliferated from tissues other than the cambium or procambium by culturing the obtained  Taxus  cambium- or procambium-containing tissue; and   (c) collecting a cell line from the cambium or procambium layer.   
     
     
         4 . The pharmaceutical composition for preventing or treating cancer according to  claim 3 , wherein the step (c) is performed by proliferating cell line in a medium, which contains 3-5 wt % of raw sugar or sugar and at least one substance selected from the group consisting of methyl jasmonate, fungal extract, bacterial extract, yeast extract, chitosan, glucomanan, glucan, phenylalanine, benzoic acid, salicylic acid, arachonic acid, STS, mevalonalonate N-benzolyglycine, ABA, SNP, IPP, BHT, CCC, ethephon, hippuic acid, amminoium ceric nitrate, AgNO 3 , vanadyl sulfate, p-aminobenzoic acid, brassinosteroids, sodium alginate, and sodium acetate, and then collecting the proliferated cell line. 
     
     
         5 . The pharmaceutical composition for preventing or treating cancer according to  claim 1 , wherein the extract is obtained using a solvent selected from the group consisting of distilled water, alcohol, acetone, DMSO (dimethyl sulfoxide), and mixed solvents thereof. 
     
     
         6 . The pharmaceutical composition for preventing or treating cancer according to any one claim among  claims 1 - 5 , wherein the cancer is selected from the group consisting of colon cancer, oral cavity carcinoma, lung cancer, prostate cancer, osteosarcoma, leukemia, uterine cancer, skin cancer, pancreas cancer, breast cancer, gastric cancer, renal cancer and liver cancer. 
     
     
         7 . A functional food for preventing or improving cancer, which contains any one or more selected from the group consisting of a cell line, which is derived from the cambium or procambium of  Taxus  and has the following characteristics; a lysate thereof; an extract thereof; and a culture medium thereof:
 (a) it is morphologically characterized by a large number of vacuoles;   (b) it is in an innately undifferentiated state; and   (c) it is a homogeneous cell line.   
     
     
         8 . The functional food for preventing or improving cancer according to  claim 7 , wherein the cell line is additionally characterized in that: (a) it is present as single cells during suspension culture; (b) it has a growth rate higher than those of cell lines derived from tissues other than the cambium or procambium of  Taxus  and is cultured stably; and (c) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium or procambium of  Taxus.    
     
     
         9 . The functional food for preventing or improving cancer according to  claim 7 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a  Taxus  cambium- or procambium-containing tissue;   (b) inducing a cambium or procambium layer proliferated from the cambium or procambium and an amorphous callus layer proliferated from portions other than the cambium or procambium by culturing the obtained  Taxus  cambium- or procambium-containing tissue; and   (c) collecting a cell line from the cambium or procambium layer.