Mammalian tumor susceptibility gene products and their uses

Abstract

The present invention provides methods and compositions for regulating ubiquitination in a cell. In particular, the present invention provides purified polypeptides comprising an ubiquitination-regulating domain. The invention also provides methods of using such polypeptides for screening for agents, for producing antibodies, and for treatment of diseases, e.g., proliferative diseases, neurodegenerative diseases, autoimmune diseases, metabolic disease and developmental abnormalities. The invention further provides antibodies that bind an ubiquitination-regulating domain and agents and antibodies that regulate ubiquitination in cells, e.g., by modulating the interaction between a TSG101 protein and an MDM2 protein.

1 - 42 . (canceled) 
     
     
         43 . A method of treating prostate cancer in a mammal caused by abnormal function of TSG101 in cells of said mammal, comprising:
 administering a therapeutically effective amount of a pharmaceutical composition comprising an antibody to TSG101 which binds to an epitope in amino acid residues 1-250 of TSG101, and a pharmaceutically acceptable excipient.   
     
     
         44 . The method of  claim 43 , wherein said mammal is a human. 
     
     
         45 . The method of  claim 43 , wherein said antibody is a monoclonal antibody.