Soybean sudden death syndrome resistant soybeans, soybean cyst nematode resistant soybeans and method of breeding and identifying resistant plants

Abstract

The invention provides a method of introgressing soybean sudden death syndrome (SDS) or soybean cyst nematode (SCN) resistance into non-resistant soybean germplasm. Loci associated with SDS resistance or with SCN resistance in soybean lines known to be resistant to SDS or to SCN are used in marker assisted selection during introgression of SDS or SCN resistance into elite germplasm. In addition, the method may be used to confirm selection of resistance in new soybean cultivars.

1 . A method of conveying soybean sudden death resistance into non-resistant soybean germplasm comprising the step of introgressing soybean sudden death resistance into non-resistant soybean germplasm using one or more nucleic acid markers for marker assisted selection among soybean lines to be used in a soybean breeding programs, said markers associated with soybean sudden death syndrome resistance. 
     
     
         2 . (canceled) 
     
     
         3 . The method of  claim 1 , wherein said marker assisted selection comprises the use of an analysis technique selected from the group consisting of RAPD analysis, RFLP analysis, microsatellite analysis, and AFLP analysis. 
     
     
         4 . The method of  claim 1 , wherein the source of the sudden death syndrome resistance comprises a soybean strain selected from the group consisting of Forrest or a descendant thereof, Essex or a descendant thereof, Pyramid or a descendant thereof, Ripley or a descendant thereof, Jack or a descendant thereof, PI520733 or a descendant thereof, PI567507B or a descendant thereof, PI567.365 or a descendant thereof, PI567.446B or a descendant thereof, PI567.373B or a descendant thereof, PI90763 or a descendant thereof, and PI209332 or a descendant thereof. 
     
     
         5 - 7 . (canceled) 
     
     
         8 . A quantitative trait locus associated with resistance to soybean sudden death syndrome, said locus mapping to a linkage group in the soybean genome, said linkage group selected from the group consisting of G, N, C2, B, D, A2, and C1. 
     
     
         9 . A quantitative trait locus of  claim 8 , said locus mapping to linkage group G and mapped by one or more of the markers OI03 450 , OI03 512 , SATT309, SATT214, SATT275, SIUSAT122, CTAAGG280, CGGAGA300, ATGCGA190, AGGCAC310, CCACCA120, CCCTC220, ACGCAT80, OG13 490 , Bng122, SATT163, SATT38, A112I, OE04 450 , OE02 1000 , and SATT130. 
     
     
         10 . A quantitative trait locus of  claim 8 , said locus mapping to linkage group N and mapped by one or more of the markers OC01 500 , OO04 1075  and SATT9. 
     
     
         11 . A quantitative trait locus of  claim 8 , said locus mapping to linkage group C2 and mapped by one or more of the markers OO05 250 , K455D and OP13 500 . 
     
     
         12 . A quantitative trait locus of  claim 8 , said locus mapped by one or more of the markers OG01 1000 , SZ19 500  and SATT71. 
     
     
         13 . A quantitative trait locus of  claim 8 , said locus mapping to linkage group A2 and mapped by one or more of the markers OW15 1000 , AO85, OA12 1000 , BLT65, CCAAGC309, CCCATG349, CCGAAC400, CCGAAC401, CCCATG350, CCAAGC310, OW 15500, and OD04 500 . 
     
     
         14 . A quantitative trait locus of  claim 8 , said locus mapping to linkage group C1 and mapped by one or more of the markers A063I and SAT42. 
     
     
         15 - 21 . (canceled) 
     
     
         22 . An improved soybean plant, seed or tissue culture produced by the method of  claim 1 . 
     
     
         23 . (canceled) 
     
     
         24 . An improved soybean plant, and parts thereof, which evidences a resistance response to soybean sudden death syndrome (SDS) comprising a genome, homozygous with respect to genetic alleles which are native to a first parent and normative to a second parent of the improved soybean plant, wherein said second parent evidences significantly less resistant response to SDS than said first parent and said improved plant comprises alleles from said first parent that evidences resistance to SDS in hybrid combination in at least one locus selected from: a locus mapping to linkage group G and mapped by one or more of the markers OI03 450 , OG13 490 , Bng122, SATT163, SATT38, A112I, OE04 450, OE 02 1000  and SATT130; a locus mapping to linkage group N and mapped by one or more of the markers OC01 500 , OO04 1075  and SATT9; a locus mapping to linkage group C2 and mapped by one or more of the markers OO05 250 , K455D and OP13 500 ; a locus mapped by one or more of the markers OG01 1000 , SZ19 500  and SATT71; a locus mapping to linkage group A2 and mapped by one or more of the markers OW15 1000 , A085 and OA12 1000 ; or a locus mapping to linkage group C1 and mapped by one or more of the markers A063I and SAT42, said resistance not significantly less than that of the first parent in the same hybrid combination, and yield characteristics which are not significantly different than those of the second parent in the same hybrid combination. 
     
     
         25 . The inbred soybean plant of  claim 24  comprising each of: a locus mapping to linkage group G and mapped by one or more of the markers OI03 450 , OG13 490 , Bng122, SATT163, SATT38, A1121, OE04 450 , OE02 1000  and SATT130; a locus mapping to linkage group N and mapped by one or more of the markers OC01 500 , OO04 1075  and SATT9; a locus mapping to linkage group C2 and mapped by one or more of the markers OO05 250 , K455D and OP13 500 ; a locus mapping to linkage group D and mapped by one or more of the markers OG01 1000 , SZ19 500  and SATT71; a locus mapping to linkage group A2 and mapped by one or more of the markers OW15 1000 , A085 and OA12 1000 ; or a locus mapping to linkage group C1 and mapped by one or more of the markers A063I and SAT42, and having improved resistance to SDS when compared to a substantially identical inbred soybean plant not comprising said loci. 
     
     
         26 . A soybean plant, and parts thereof, as claimed in  claim 24  comprising the progeny of a cross between first and second inbred lines, alleles conferring resistance to sudden death syndrome (SDS) being present in the homozygous state in the genome of one or the other or both of said first and second inbred lines such that the genome of said first and second inbreds together donate to the hybrid a complement of alleles necessary to confer the resistance to SDS. 
     
     
         27 . A sudden death syndrome resistant hybrid and parts thereof formed with the improved soybean plant according to  claim 25 . 
     
     
         28 . A soybean plant and parts thereof formed by selfing the sudden death syndrome resistant hybrid of  claim 27 . 
     
     
         29 . (canceled) 
     
     
         30 . A method for identifying soybean sudden death syndrome (SDS) resistance or soybean cyst nematode resistance in a soybean plant using a SDS resistance gene, a SCN resistance gene or DNA segments having homology to a SDS resistance gene or to a SCN resistance gene, the method comprising the steps of:
 (a) probing nucleic acids obtained from the soybean plant with a probe derived from said SDS resistance gene or from said SCN resistance gene or from said DNA segment having homology to said SDS resistance gene or to said SCN resistance gene; and   (b) observing hybridization of said probe to said nulceic acids, the presence of said hybrization indicating SDS or SCN resistance in said soybean plant.   
     
     
         31 . The method of  claim 30 , further comprising using the probe derived from a known resistance gene to probe plant nucleic acids to look for mapping of the probe to loci for SDS or SCN resistance, the presence of said mapping indicating the presence of SDS or SCN resistance. 
     
     
         32 . (canceled) 
     
     
         33 . A quantitative trait locus associated with resistance to soybean cyst nematode, said locus mapping to linkage group B and mapped by one or more of the markers OG01 1000 , SZ19 500  and SATT71. 
     
     
         34 . (canceled) 
     
     
         35 . An improved soybean plant having soybean cyst nematode (SCN) resistance, the genome of which contains genes associated with said SCN resistance at a locus mapping to linkage group B and mapped by one or more of the markers OG01 1000 , SZ19 500  and SATT71. 
     
     
         36 . An isolated and purified soybean rfs1 gene, said gene capable of conveying  Fusarium solani -infection resistance to a non-resistant soybean germplasm, said gene located within a quantitative trait locus mapping to linkage group G and mapped by genetic markers OI03 512  and Bng122, said gene located along said quantitative trait locus between said markers. 
     
     
         37 . An isolated and purified soybean rft1 gene, said gene capable of conveying  Fusarium solani  toxin resistance to a non-resistant soybean germplasm, said gene located within a quantitative trait locus mapping to linkage group G and mapped by genetic markers OI03 512  and SATT309, said gene located along said quantitative trait locus between said markers. 
     
     
         38 . A method of determining soybean sudden death syndrome resistance in a soybean plant in a greenhouse setting, the method comprising the steps of:
 (a) inoculating soil with a low density inoculum of  Fusarium solani;      (b) planting a soybean plant in said inoculated soil;   (c) growing said plant in said soil for a predetermined period of time in a greenhouse;   (d) isolating  Fusarium solani -infected tissue from said plant;   (e) culturing said infected tissue for a period of time sufficient to allow for fungal colony forming unit growth;   (f) statistically determining disease severity and/or infection severity in said plant using the number of said fungal colony forming units; and   (g) comparing said disease severity and/or said infection severity to disease severity and/or infection severity data from genetic markers associated with soybean sudden death syndrome resistance to identify a correlation, the presence of a statistically significant correlation indicating the presence of soybean sudden death syndrome resistance in said soybean plant.   
     
     
         39 . The method of  claim 38 , wherein said low density inoculum of  Fusarium solani  comprises an inoculum density of about 3×10 3  spore/cm 3  soil. 
     
     
         40 . The method of  claim 38 , wherein said  Fusarium solani -infected plant tissue comprises root tissue. 
     
     
         41 - 42 . (canceled)