Compositions and methods for quantification of serum glycoproteins

Abstract

The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins.

1 - 40 . (canceled) 
     
     
         41 . A composition comprising a plurality of peptides containing the glycosylation sites referenced as SEQ ID NOS: 1-3482, wherein said peptides each correspond to peptide fragments derived by cleavage of polypeptides using the same cleavage reagent. 
     
     
         42 . The composition of  claim 41 , wherein said cleavage reagent is a protease. 
     
     
         43 . The composition of  claim 42 , wherein said protease is trypsin. 
     
     
         44 . A kit comprising a plurality of peptides containing the glycosylation sites sh referenced as SEQ ID NOS: 1-3482, wherein said peptides each correspond to peptide fragments derived by cleavage of polypeptides using the same cleavage reagent. 
     
     
         45 . The kit of  claim 44 , further comprising a pair of differentially labeled isotope tags. 
     
     
         46 . The kit of  claim 44 , further comprising the cleavage reagent corresponding to said peptide fragments. 
     
     
         47 . The kit of  claim 46 , wherein said cleavage reagent is a protease. 
     
     
         48 . The kit of  claim 47 , wherein said protease is trypsin. 
     
     
         49 . The kit of  claim 44 , further comprising a hydrazide resin. 
     
     
         50 . The kit of  claim 44 , further comprising a glycosidase.