Purification of adenovirus and aav

Abstract

The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.

1 . A method of purifying active and infectious adenovirus comprising the step of contacting said virus with a chromatographic matrix material having binding groups with affinity for said virus and wherein said binding groups are confined to pores in said matrix sufficiently large to allow passage of said virus there through in undamaged form. 
     
     
         2 . A method of purifying active and infectious adenovirus comprising the step of contacting said virus with a chromatographic matrix material comprising pores and having binding groups with affinity for said virus wherein said matrix further comprises crosslinking or tentacles sufficient to substantially prevent the virus from contacting said pores thereof. 
     
     
         3 . A method of purifying adeno-associated virus from a sample also containing adenovirus comprising the step of contacting said sample with a chromatographic material capable of damaging adenovirus, wherein said damaged adenovirus becomes non-infectious. 
     
     
         4 . A method of purifying adeno-associated virus substantially in conformity with the disclosure herein.