Glycine N-methyltransferase (GNMT) Animal model and use thereof

Abstract

The present invention is a new type of Glycine N-methyltransferase (GNMT) knockout mice model. This model can be applied to screen drug, test of treatment and search for diagnostic marker of hepatocellular carcinoma (HCC), glycogen storage disease, liver dysplasia, fatty liver and other liver disease.

1 . A pair of prime, which is (i) SEQ ID Nos 1 and 2 or (ii) SEQ ID Nos 3 and 4. 
     
     
         2 . A database for regulatory genes in GNMT knock-out mouse comprising up-regulatory genes as listed in Tables 3-1 and 3-2 and down-regulatory genes as listed in Tables 3-1, 3-2 and 4. 
     
     
         3 . A database for hepatocellular carcinoma signaling pathway genes comprising
 (a) survival and proliferation: PTEN PI3K Akt 1 GSK3β or β-catenin;   (b) oncogenes: Cyclin D1 C-myc or C-Jun; and   (c) tumor suppressor gene: Rb or p53.