Cell Culture Flask and the Cell Culture Device Having it

Abstract

The present invention relates to a cell culture flask for culturing a cell using a culture solution, particularly to a cell culture flask enabling to culture a cell without any contamination, to automatically introduce and discharge the culture solution or gases and to be stacked in turn, and a cell culture device having the same. The present invention provides a cell culture flask comprising a culture space with a predetermined area for culturing the cell; one or more culture solution inlet ports for introducing the culture solution or the cell into the culture space; one or more culture solution outlet ports for discharging the culture solution or the cell supplied to the culture space; and one or more gas inlet ports for introducing the various gases into the culture space, wherein the cell culture flask is made airtight and formed of a transparent material so that a user can see an internal portion of the cell culture flask, and a cell culture device having the same.

1 . A cell culture flask for culturing a cell using a culture solution and various gases, comprising:
 a culture space with a predetermined area for culturing the cell;   one or more culture solution inlet ports for introducing the culture solution or the cell into the culture space;   one or more culture solution outlet ports for discharging the culture solution or the cell supplied to the culture space; and   one or more gas inlet ports for introducing the various gases into the culture space,   wherein the cell culture flask is made airtight and formed of a transparent material so that a user can see an internal portion of the cell culture flask.   
     
     
         2 . The cell culture flask according to  claim 1 , wherein the culture solution inlet port and the culture solution outlet port comprises:
 a cylindrical tube member which is outwardly protruded to receive the culture solution and formed with a hole through which the culture solution is flowed;   a piston member which is disposed to be reciprocated in the cylindrical tube member;   a connection member of which one end is connected with the piston member and the other end is connected with an external power supply part; and   a sealing member which is attached to an end of the cylindrical tube member and formed with an opening through which the connection member can be reciprocated.   
     
     
         3 . The cell culture flask according to  claim 2 , wherein the power supply part comprises a detachable motor operated by electric power supplied through a cable from an outside, and the connection member comprises a linear rod or a lead screw. 
     
     
         4 . The cell culture flask according to  claim 1 , wherein the cell culture flask further comprises one or more gas outlet ports for discharging the various gases supplied to the culture space. 
     
     
         5 . The cell culture flask according to  claim 4 , wherein the cell culture flask further comprises one or more foreign substance inlet ports for introducing a foreign substance. 
     
     
         6 . The cell culture flask according to  claim 5 , wherein the foreign substance inlet port is opened and closed by a cap-type stopper. 
     
     
         7 . The cell culture flask according to  claim 6 , wherein the cell culture flask is formed into a cylindrical shape with a predetermined height and comprises the culture space at a center portion thereof, and the culture solution inlet and outlet ports, the gas inlet and output ports, and the foreign substance inlet ports are formed along a side surface of the cell culture flask. 
     
     
         8 . The cell culture flask according to  claim 1 , wherein the transparent material comprises a plastic such as lexan and acryl or a tempered glass. 
     
     
         9 . A cell culture device, comprising:
 a cell culture flask comprising a culture space for culturing cells, a culture solution inlet port for introducing a culture solution or the cell into the culture space, a culture solution outlet port for discharging the culture solution or the cell supplied to the culture space, and a gas inlet port for introducing various gases into the culture space, wherein the cell culture flask is made airtight and formed of a transparent material to observe its internal portion;   an injection unit for supplying the culture solution to the cell culture flask through a culture solution inlet port of the cell culture flask;   a collection unit for collecting the culture solution to outside of the cell culture flask through a culture solution outlet port of the cell culture flask; and   a cell culture flask receiving portion comprising an injecting part and a collecting part to be equipped with the injection unit and the collecting unit, a plate-shaped connecting part which separately connects an upper part or a lower part of the collecting part and the injecting part, and a supporting part which is provided at the connecting part,   wherein the cell culture flask is disposed between the connecting parts of the cell culture flask receiving portion.   
     
     
         10 . The cell culture device according to  claim 9 , wherein the cell culture device further comprises one or more gas outlet ports for discharging the various gases supplied to the culture space. 
     
     
         11 . The cell culture device according to  claim 10 , wherein the cell culture device further comprises one or more foreign substance inlet ports for introducing the foreign substance. 
     
     
         12 . The cell culture device according to  claim 11 , wherein the foreign substance inlet port is opened and closed by a cap-type stopper. 
     
     
         13 . The cell culture device according to  claim 12 , wherein the cell culture flask is formed of a cylindrical shape with a predetermined height comprising the culture space at a center portion thereof, and the culture solution inlet and outlet ports, the gas inlet and output ports, and the foreign substance inlet ports are formed along the side surface of the cell culture flask. 
     
     
         14 . The cell culture device according to  claim 9 , wherein the transparent material comprises a plastic such as lexan and acryl or a tempered glass. 
     
     
         15 . The cell culture device according to  claim 9 , wherein the injection unit and the collection unit comprises:
 a syringe part comprising a power supply part;   a culture solution storing part which is connected with the syringe part and stores the culture solution;   a contact part which is connected with the culture solution storing part and contacted with the culture solution inlet port or the culture solution outlet port of the cell culture flask to flow the culture solution;   a piston member which is disposed to be reciprocated in the culture solution storing part; and   a connection member of which one end is connected with the piston member and the other end is connected with the power supply part,   wherein the injection unit and the collection unit are formed into an injector as a whole.   
     
     
         16 . The cell culture device according to  claim 15 , wherein the connection member comprises a linear rod or a lead screw shape. 
     
     
         17 . The cell culture device according to  claim 15 , wherein the power supply part comprises a detachable motor which is operated by electric power supplied through a cable from an outside. 
     
     
         18 . The cell culture device according to  claim 9 , wherein the injecting part and the collecting part of the cell culture flask receiving portion are radially or vertically connected with an outer surface of the cell culture flask. 
     
     
         19 . The cell culture device according to  claim 9 , wherein the cell culture flask receiving portion is formed of a plastic such as lexan and acryl or a stainless steel.