Nucleic acids isolated from the intestine

Abstract

A recombinant intestinal nucleic acid is provided. One embodiment of the present invention contemplates the use of a gut-specific promoter, wherein a promoter can be the chicken intestinal fatty acid binding protein promoter region. A method for making a transgenic bird is also disclosed by transfecting a bird with a vector comprising a recombinant nucleic acid comprising a chicken intestinal fatty acid binding protein promoter region operably linked to a heterologous nucleic acid expressing a desired polypeptide to be expressed in the gut tissue of an avian.

1 . An isolated nucleic acid comprising a nucleotide sequence having at least 95% identity to SEQ ID NO: 2. 
     
     
         2 . The isolated nucleic avid of  claim 1  having at least 99% identity to SEQ ID NO: 2. 
     
     
         3 . The isolated nucleic acid of  claim 1  wherein comprising the sequence of SEQ ID NO: 2. 
     
     
         4 . The isolated nucleic acid of  claim 1  further comprising a nucleotide sequence encoding a polypeptide. 
     
     
         5 . The isolated nucleic acid of  claim 4  wherein the nucleotide sequence encoding a polypeptide is codon optimized for protein expression in an avian. 
     
     
         6 . The isolated nucleic acid of  claim 1  further comprising a polyadenylation signal sequence. 
     
     
         7 . The isolated nucleic acid of  claim 6  wherein the polyadenylation signal sequence is en SV40 virus polyadenylation signal sequence. 
     
     
         8 . The isolated nucleic acid of  claim 1  further comprising a vector. 
     
     
         9 . The isolated nucleic acid of  claim 8  wherein the vector is a virus. 
     
     
         10 . An expression vector comprising a nucleotide sequence having at least 95% identity to SEQ ID NO: 2. 
     
     
         11 . The expression vector of  claim 10  comprising a nucleotide sequence having at least 99% identity SEQ ID NO: 2. 
     
     
         12 . The expression vector of  claim 10  comprising the sequence of SEQ ID NO: 2. 
     
     
         13 . The expression vector of  claim 10  comprising a nucleotide sequence encoding a polypeptide. 
     
     
         14 . The expression vector of  claim 10  further comprising an origin of replication. 
     
     
         15 . A method of expressing a polypeptide in a host cell comprising: introducing into a host cell a nucleic acid comprising a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 2 operably linked to a nucleotide sequence encoding a polypeptide; and maintaining the host cell under conditions suitable for expression of the polypeptide under the control of the gene expression control region. 
     
     
         16 . The method of  claim 15  wherein the gene expression controlling region comprises a nucleotide sequence having at least 99% identity to SEQ ID NO: 2. 
     
     
         17 . The method of  claim 15  wherein the gene expression controlling region comprises SEQ ID NO: 2. 
     
     
         18 . The method of  claim 15  wherein the nucleotide sequence encoding a polypeptide is codon optimized for protein expression in an avian. 
     
     
         19 . The method of  claim 15  wherein the nucleic acid further comprises a polyadenylation signal sequence. 
     
     
         20 . The method of  claim 15  wherein the nucleic acid molecule further comprises a vector.