US2010317063A1PendingUtilityA1

COMPOSITIONS FOR IMPROVED cDNA SYNTHESIS

Assignee: LIFE TECHNOLOGIES CORPPriority: Mar 2, 1999Filed: Nov 21, 2009Published: Dec 16, 2010
Est. expiryMar 2, 2019(expired)· nominal 20-yr term from priority
C12N 15/1096C12P 19/34C07K 16/40
72
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Claims

Abstract

The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.

Claims

exact text as granted — not AI-modified
1 . A method for synthesizing one or more cDNA molecules or population of cDNA molecules comprising:
 mixing at least one mRNA or poly A RNA template or population of such templates with at least one polypeptide having reverse transcriptase activity; and   incubating said mixture under conditions sufficient to increase the amount or percentage of full-length cDNA molecules synthesized.

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