Transcription chip

Abstract

A transcription chip comprising a substrate and, immobilized thereon, at least one polynucleotide including an element sequence to which a transcription factor can be bound.

1 . A transcription chip wherein at least one polynucleotide comprising one or more element sequences to which a transcription factor can be bound is immobilized on a substrate. 
     
     
         2 . The chip according to  claim 1  wherein at least two polynucleotides comprising one or more element sequences to which transcription factors can be bound respectively are bound. 
     
     
         3 . The chip according to  claim 1  wherein said polynucleotide has a partial sequence of a promoter in the upstream (5′) side of each gene. 
     
     
         4 . The chip according to  claim 1  wherein said substrate is made by forming a diamond thin film on a support. 
     
     
         5 . The chip according to  claim 1  wherein said polynucleotide is bound to the diamond thin film through an optionally appropriate spacer. 
     
     
         6 . A method for assaying a binding of a transcription factor comprising a step of interacting a sample capable of comprising the transcription factor with the transcription chip according to  claim 1 . 
     
     
         7 . The method according to  claim 6  for evaluating an effect of a subject substance on the transcription factor, wherein said sample is a cell lysate of cells cultured in the presence of the subject substance. 
     
     
         8 . The method according to  claim 7  wherein the binding of the transcription factor is detected by an antibody against the transcription factor or a method utilizing mass spectrometry. 
     
     
         9 . The method according to  claim 8  wherein the detection using the antibody is an ELISA and the method utilizing mass spectrometry is a peptide mass fingerprint method.