Method of fixing and expressing physiologically active substance

Abstract

The present invention provides methods for retaining and expressing physiologically active substances in a target tissue-specific-manner, by administering the physiologically active substances to target submucous tissue. Specifically, the present inventors demonstrated that, when physiologically active substances were directly administered into submucous tissues without using a carrier, the physiologically active substances were effectively and safely retained at the administration sites over long periods without loss and diffusion, and produced the effect acting in a reservoir-like fashion. The physiologically active substances administered as described above were demonstrated to produce the therapeutic effect without having an influence on organs other than the administered organ.

1 . A method for retaining and expressing a physiologically active substance in a target submucous tissue-specific manner, wherein the physiologically active substance is administered into the submucous tissue. 
     
     
         2 . The method of  claim 1 , wherein the physiologically active substance is selected from nucleic acids, proteins, carbohydrates, lipids, or low-molecular-weight compounds. 
     
     
         3 . The method of  claim 2 , wherein the nucleic acid is an siRNA. 
     
     
         4 . The method of any one of  claims 1  to  3 , wherein the physiologically active substance is administered in combination with a pharmaceutically acceptable carrier.