Compositions for delivery of cargo such as drugs proteins and/or genetic materials

Abstract

Compositions and methods for delivery of cargo molecules to a patient or subject in need thereof include a proteoliposome carrier vehicle that incorporates an RLIP76 protein and contains the cargo molecule. The vehicle effectively delivers the cargo molecule systemically throughout the tissues of the body including into the central nervous system.

1 . A method of delivering a cargo molecule to a cell, comprising contacting said cell with a proteoliposome comprising an RLIP76 protein and a cargo molecule. 
     
     
         2 . The method of  claim 1 , wherein the cell is a brain, heart, liver, kidney, lung, or eye cell. 
     
     
         3 . The method of  claim 3 , wherein the cell is a brain cell. 
     
     
         4 . The method of  claim 1 , wherein the cell is a tumor cell. 
     
     
         5 . The method of  claim 4 , wherein the cell is a brain tumor cell. 
     
     
         6 . The method of  claim 1 , wherein the cargo molecule is a nucleic acid molecule or a polypeptide molecule. 
     
     
         7 . The method of  claim 1 , wherein the cargo molecule is a nucleic acid molecule. 
     
     
         8 . The method of  claim 7 , wherein the cargo molecule is a RNA molecule. 
     
     
         9 . The method of  claim 8 , wherein the cargo molecule is an antisense RNA molecule. 
     
     
         10 . The method of  claim 8 , wherein the cargo molecule is a small inhibitory RNA molecule. 
     
     
         11 . A composition comprising a proteoliposome comprising an RLIP76 protein and a cargo molecule, wherein the proteoliposome is in a pharmaceutically acceptable carrier. 
     
     
         12 . The composition of  claim 11 , wherein the cargo molecule is a nucleic acid molecule or a polypeptide molecule. 
     
     
         13 . The composition of  claim 12 , wherein the cargo molecule is a nucleic acid molecule. 
     
     
         14 . The composition of  claim 13 , wherein the cargo molecule is an antisense nucleic acid molecule. 
     
     
         15 . The composition of  claim 13 , wherein the cargo molecule is a small inhibitory ribonucleic acid molecule. 
     
     
         16 . The composition of  claim 11 , wherein the pharmaceutically acceptable carrier is formulated for oral, intraperitoneal, intravenous, intramuscular, inhalation, transmucosal or transdermal administration. 
     
     
         17 . The composition of  claim 16 , wherein the pharmaceutically acceptable carrier is formulated for oral administration. 
     
     
         18 . A method of delivering a cargo molecule to a subject in need thereof comprising administering to the subject a therapeutically effective amount of a pharmaceutical composition comprising a proteoliposome in a pharmaceutically acceptable carrier, wherein the proteoliposome comprises an RLIP76 protein and the cargo molecule. 
     
     
         19 . The method of  claim 18 , wherein the cargo molecule is a nucleic acid molecule or a polypeptide molecule. 
     
     
         20 . The method of  claim 19 , wherein the cargo molecule is a nucleic acid molecule. 
     
     
         21 . The method of  claim 20 , wherein the cargo molecule is an antisense nucleic acid molecule. 
     
     
         22 . The method of  claim 21 , wherein the cargo molecule is a small inhibitory ribonucleic acid molecule. 
     
     
         23 . The method of  claim 18 , wherein the pharmaceutical composition is delivered orally, intraperitoneally, intravenously, intramuscularly, by inhalation, transmucosally or transdermally. 
     
     
         24 . The method of  claim 23 , wherein the pharmaceutical composition is delivered orally. 
     
     
         25 . The method of  claim 18 , wherein the cargo molecule is delivered to the brain, heart, liver, kidney or lung of the subject. 
     
     
         26 . The method of  claim 25 , wherein the cargo molecule is delivered to the brain. 
     
     
         27 . The method of  claim 26 , wherein the cargo molecule is delivered to neurons or endothelial cells of blood vessels within the brain. 
     
     
         28 . The method of  claim 27 , wherein the cargo molecule is delivered to neurons and endothelial cells of blood vessels within the brain. 
     
     
         29 . The method of  claim 18 , wherein the cargo molecule is delivered to a tumor within the subject. 
     
     
         30 . The method of  claim 29 , wherein the cargo molecule is delivered to a tumor within the central nervous system of the subject. 
     
     
         31 . The method of  claim 18 , wherein the cargo molecule is delivered to a muscle of the subject.