US2011020526A1PendingUtilityA1

Corn plants and seed enhanced for asparagine and protein

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Assignee: FABBRI BRADON JPriority: May 16, 2005Filed: Jan 26, 2010Published: Jan 27, 2011
Est. expiryMay 16, 2025(expired)· nominal 20-yr term from priority
Inventors:Bradon J. FabbriBo-Xing QiuSteven ScreenScott E. AndersenJames H. Crowley
C12N 9/93C12N 15/8251Y02A40/146
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Claims

Abstract

The present invention relates to a corn plant and seed with enhanced levels of protein and amino acids. The invention also relates to DNA constructs that provide expression in transgenic corn cells of an asparagine synthetase enzyme. The DNA constructs are used in a method to produce transgenic corn plants and seeds and to select for plants and seeds with enhanced levels of protein and amino acids.

Claims

exact text as granted — not AI-modified
1 . A transgenic corn seed with an increased protein level comprising in its genome a heterologous DNA construct comprising a promoter operably linked to a polynucleotide encoding a heterologous asparagine synthetase polypeptide, wherein said seed has an increased protein level relative to the protein level of a seed of the same variety not containing said DNA construct in its genome. 
     
     
         2 . The transgenic corn seed of  claim 1 , wherein said polynucleotide comprises a nucleic acid sequence selected from the group consisting of:
 (a) a nucleic acid sequence comprising the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17;   (b) a nucleic acid sequence that encodes the polypeptide sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8; and   (c) a nucleic acid sequence that hybridizes to the sequence of (a) or (b) or a complement thereof under high stringency conditions of about 0.2×SSC and 65° C.   
     
     
         3 . The transgenic corn seed of  claim 1 , wherein said heterologous asparagine synthetase polypeptide comprises SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8. 
     
     
         4 . The transgenic corn seed of  claim 1 , wherein said polynucleotide comprises the nucleic acid sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17. 
     
     
         5 . The transgenic corn seed of  claim 1 , wherein said heterologous asparagine synthetase polypeptide is a corn AsnS2 polypeptide. 
     
     
         6 . The transgenic corn seed of  claim 1 , wherein said promoter is a rice actin 1 promoter. 
     
     
         7 . A transgenic corn plant with an increased asparagine level comprising in its genome a heterologous DNA construct comprising a promoter operably linked to a polynucleotide encoding a heterologous asparagine synthetase, wherein said plant has increased asparagine level relative to the asparagine level of a plant of the same variety not containing said DNA construct in its genome. 
     
     
         8 . The transgenic corn plant of  claim 7 , wherein said polynucleotide encoding a heterologous asparagine synthetase comprises a nucleic acid sequence selected from the group consisting of:
 (a) a nucleic acid sequence comprising the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17;   (b) a nucleic acid sequence that encodes the polypeptide sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8; and   (c) a nucleic acid sequence that hybridizes to the sequence of (a) or (b) or a complement thereof under high stringency conditions of about 0.2×SSC and 65° C.   
     
     
         9 . The transgenic corn plant of  claim 7 , wherein said asparagine synthetase comprises SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8. 
     
     
         10 . The transgenic corn plant of  claim 7 , wherein said polynucleotide comprises the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17. 
     
     
         11 . The transgenic corn plant of  claim 7 , wherein said promoter is a rice actin 1 promoter. 
     
     
         12 . A method of producing a transgenic corn plant with increased asparagine comprising
 a) transforming a corn cell with a heterologous DNA construct comprising a promoter molecule functional in a corn cell operably linked to a DNA molecule encoding an asparagine synthetase polypeptide;   b) regenerating the corn cell into and intact corn plant;   c) selecting a corn plant that has increased asparagine in a tissue relative to a corn plant tissue not transformed with said DNA construct;   d) growing the corn plant to maturity; and   e) harvesting a seed from the corn plant.   
     
     
         13 . The method of  claim 12 , wherein said DNA molecule comprises a nucleic acid sequence selected from the group consisting of:
 (a) a nucleic acid sequence comprising the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17;   (b) a nucleic acid sequence that encodes the polypeptide sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8; and   (c) a nucleic acid sequence that hybridizes to the sequence of (a) or (b) or a complement thereof under high stringency conditions of about 0.2×SSC and 65° C.   
     
     
         14 . The method of  claim 12 , wherein said asparagine synthetase polypeptide comprises the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8. 
     
     
         15 . The method of  claim 12 , wherein said DNA molecule comprises the nucleic acid sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17. 
     
     
         16 . The method of  claim 12 , further comprising the step of:
 f) selecting a seed with increased protein.   
     
     
         17 . A corn meal with increased protein relative to other corn meals, wherein the corn meal comprises a heterologous DNA construct comprising a promoter molecule operably linked to a DNA molecule encoding a heterologous asparagine synthetase polypeptide. 
     
     
         18 . The corn meal of  claim 17 , wherein said corn asparagine synthetase polypeptide is a corn AsnS2 polypeptide. 
     
     
         19 . The corn meal of  claim 17 , wherein said DNA molecule comprises the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID 5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17. 
     
     
         20 . The corn meal of  claim 17 , wherein said DNA molecule encodes a polypeptide of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8. 
     
     
         21 . An animal feed made from the corn meal of  claim 16 .

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