Immunovectors which can be used for the intracellular and intranuclear transport

Abstract

A method of transferring a biologically active principle of interest into a cell by coupling an antibody or a fragment of an antibody, which recognizes an epitope contained in a nucleic acid, with the biologically active principle, to form a coupled biologically active principle; and incubating the coupled biologically active principle, which is transferred through the cell membrane and into the cell, with the cell.

1 . A method of transferring a biologically active principle of interest into a cell comprising:
 a. coupling an antibody or a fragment of an antibody, which recognizes an epitope contained in a nucleic acid, with the biologically active principle, thereby forming a coupled biologically active principle; and   b. incubating the coupled biologically active principle with the cell, wherein said coupled principle is transferred through the cell membrane and into the cell.   
     
     
         2 . The method of  claim 1 , wherein said biologically active principle is selected from the group consisting of a hapten having biological activity, a hormone, and a medicine. 
     
     
         3 . The method of  claim 2 , wherein said biologically active principle is a medicine allowing the immortalization of selected type cells including one of macrophages, dendritic cells, B cells, T cells, and hematopoietic cells. 
     
     
         4 . The method of  claim 1 , wherein said biologically active principle is an antiviral agent. 
     
     
         5 . The method of  claim 1 , wherein the antibody or fragment of antibody is a monoclonal IgG, a (Fab′) 2  fragment, or a (Fab′) fragment. 
     
     
         6 . The method of  claim 1 , wherein the antibody or fragment of antibody is obtained from individuals presenting auto-immune syndromes. 
     
     
         7 . The method of  claim 1 , wherein the antibody or fragment of antibody is obtained from individuals presenting disseminated erythematous lupus syndromes. 
     
     
         8 . A hybridoma deposited at the CNCM on Jun. 30, 1996 under the registration number I-1605, I-1606 or I-1607.