Desiccated Biologics And Methods Of Preparing The Same

Abstract

The present invention provides compositions comprising desiccated biologics comprising a cell, protein, virus, nucleic acid, carbohydrate, or lipid, or any combination thereof, along with at least one membrane penetrable sugar, and at least one membrane impenetrable sugar, wherein the moisture content is from 5% to 95%, and to methods of preparing the same, and to methods of treating animals using the same.

1 . A composition comprising:
 one or more biologics;   one or more membrane penetrable sugars; and   one or more membrane impenetrable sugars;   wherein the moisture content of the composition is from about 5% to about 95%.   
     
     
         2 . The composition of  claim 1  wherein the biologic is a cell. 
     
     
         3 . The composition of  claim 2  wherein the cell is anucleated. 
     
     
         4 . The composition of  claim 3  wherein the anucleated cell is a platelet or red blood cell. 
     
     
         5 . The composition of  claim 2  wherein the cell is nucleated. 
     
     
         6 . The composition of  claim 5  wherein the nucleated cell is a white blood cell, a stem cell, a stem cell progenitor cell, a gamete, a gamete progenitor cell, a hepatocyte, a muscle cell, a kidney cell, an endothelial cell, an epithelial cell, an erythroblast, a leukoblast, a chondroblast, or a pancreatic cell. 
     
     
         7 . The composition of  claim 1  wherein the biologic is a protein, virus, nucleic acid, carbohydrate, or lipid. 
     
     
         8 . The composition of  claim 1  wherein the membrane penetrable sugar is trehalose. 
     
     
         9 . The composition of  claim 1  wherein the membrane impenetrable sugar is dextran. 
     
     
         10 . The composition of  claim 1  wherein the moisture content is from about 15% to about 40%. 
     
     
         11 . The composition of  claim 1  wherein the moisture content is from about 20% to about 25%. 
     
     
         12 . The composition of  claim 1  wherein the biologic is a platelet, the membrane penetrable sugar is trehalose, the membrane impenetrable sugar is dextran, and the moisture content is about 15%. 
     
     
         13 . The composition of  claim 1  wherein the biologic is a red blood cell, the membrane penetrable sugar is trehalose, the membrane impenetrable sugar is dextran, and the moisture content is about 25%. 
     
     
         14 . The composition of  claim 1  wherein the biologic is a white blood cell, the membrane penetrable sugar is trehalose, the membrane impenetrable sugar is dextran, and the moisture content is about 50%. 
     
     
         15 - 34 . (canceled)