Microginin producing proteins and nucleic acids encoding a microginin gene cluster as well as methods for creating novel microginins

Abstract

The invention provides for nucleic acid molecules enabling the synthesis of microginin and microginin analogues. The invention also provides for methods for identifying microginins as well creating microginins which may not be found in nature.

1 - 19 . (canceled) 
     
     
         20 . A nucleic acid encoding a peptide spacer sequence (SP), wherein
 a. the peptide sequence comprises at least 4 glycine amino acids per single repeat unit (SRU), or   b. at least five proline and/or leucine amino acids per single repeat unit (SRU),   c. a SRU within the SP is between 7 and 15 amino acids in length, and   d. the SP comprises between 2 and 10 SRUs.   
     
     
         21 . The nucleic acid according to  claim 20 , encoding a peptide SRU with a sequence as shown in SEQ ID NO. 20 or SEQ ID NO. 21. 
     
     
         22 . The nucleic acid according to  claim 21 , with a sequence as shown in SEQ ID NO. 43 or SEQ ID NO. 44.