Compositions and Methods for Cartilage Repair

Abstract

Autologous compositions and methods are provided for cartilage repair in patients in need thereof. Some aspects include combinations of platelet-based materials with chondrogenesis inducing agents in the presence or absence of cell-based therapies.

1 . A composition for facilitating cartilage repair in a patient in need thereof comprising a therapeutically effective amount of an autologous platelet lysate and one or more chondrogenesis inducing agents. 
     
     
         2 . The composition of  claim 1  wherein the platelet lysate is from about 5% to about 40% platelet lysate solution. 
     
     
         3 . The composition of  claim 1  wherein the chondrogenesis inducing agent is one or more growth factors, one or more cytokines, one or more hormones, or one or more essential nutrients. 
     
     
         4 . The composition of  claim 3  wherein the chondrogenesis inducing agent is a hormone and wherein the hormone is corticosteroid. 
     
     
         5 . The composition of  claim 3  wherein the chondrogenesis inducing agent is a growth factor and wherein the growth factor is transforming growth factor β, fibroblast growth factor or a bone morphogenetic protein. 
     
     
         6 . The composition of  claim 3  wherein the chondrogenesis inducing agent is a cytokine and wherein the cytokine is IL-1. 
     
     
         7 . The composition of  claim 3  wherein the chondrogenesis inducing agent is an essential nutrient and wherein the essential nutrient is ascorbic acid, collagen, amino acids, peptides, sodium pyruvate, glucose, glutamine, ribonucleosides, carbohydrates, essential oils or deoxyribonucleosides. 
     
     
         8 . The composition of  claim 1  further comprising platelet rich plasma, platelet rich fibrin, whole cell platelets or a mixture thereof. 
     
     
         9 . The composition of  claim 1  further comprising a gel, hydrogel, foam, or other carrier material. 
     
     
         10 . A method for facilitating cartilage repair in a patient in need thereof comprising:
 obtaining platelets from the patient;   preparing a 5% to 40% platelet lysate solution from the platelets;   obtaining one or more chondrogenesis inducing agents; and   administering the 5% to 40% platelet lysate solution and chondrogenesis inducing agent to a site in the patient in need of cartilage repair;   wherein the combined administration of the 5 to 40% platelet lysate solution and chondrogenesis inducing agent facilitates cartilage repair at the site in the patient in need of repair.   
     
     
         11 . The method of  claim 10  wherein the chondrogenesis inducing agent is one or more growth factors, one or more cytokines, one or more hormones, or one or more essential nutrients. 
     
     
         12 . The method of  claim 10  further comprising administering a platelet rich plasma, platelet rich fibrin, whole cell platelet or a mixtures thereof to the patient in need thereof wherein the administration is at the site in the patient in need of repair.