Novel hydrogels and uses thereof

Abstract

The present invention provides novel hydrogels and methods of making and using such hydrogels. The present invention provides hydrogels that may be formed by the self-assembly of peptides in solution. Such self-assembly may be brought about by a change in one or more characteristics of the solution. Characteristics of the solution that may be changed include pH, ionic strength, temperature, and concentration of one or more specific ions. In addition, hydrogels of the invention may be disassembled by changing one or more characteristic of the hydrogel such as pH, ionic strength, temperature, and concentration of one or more specific ions.

1 . An isolated peptide comprising the sequence VKVKVKVKV D PPTKVEVKVKV-NH 2 . 
     
     
         2 . A method of making a hydrogel, comprising:
 providing a solution comprising the peptide of  claim 1 ; and   altering one or more characteristics of the solution, wherein a hydrogel is formed.   
     
     
         3 . The method of  claim 2 , wherein the characteristic is selected from the group consisting of ionic strength, temperature, concentration of a specific ion, and pH. 
     
     
         4 . The method of  claim 2 , wherein altering comprises contacting the solution with electromagnetic radiation. 
     
     
         5 . The method of  claim 2 , wherein altering comprises changing the pH of the solution. 
     
     
         6 . The method of  claim 2 , wherein altering one or more characteristic of the solution results in a salt concentration of from about 20 mM to about 400 mM. 
     
     
         7 . The method of  claim 6 , wherein the salt is NaCl. 
     
     
         8 . The method of  claim 2 , wherein the solution has a pH of less than 9. 
     
     
         9 . The method of  claim 2 , wherein the solution has a pH of from about 6.0 to about 8.5. 
     
     
         10 . The method of  claim 2 , wherein the solution has a pH of from about 7.0 to about 8.0. 
     
     
         11 . The method of  claim 2 , wherein the solution has a pH of about 7.4. 
     
     
         12 . A hydrogel comprising the peptide of  claim 1 . 
     
     
         13 . The hydrogel of  claim 12 , further comprising a salt. 
     
     
         14 . The hydrogel of  claim 13 , wherein the salt is NaCl. 
     
     
         15 . A method of making a hydrogel, comprising:
 injecting a solution into an animal, wherein the solution forms a hydrogel inside the animal and wherein the solution comprises the peptide of  claim 1 .   
     
     
         16 . The method of  claim 15 , wherein the animal is a mammal. 
     
     
         17 . The method of  claim 15 , wherein the animal is a human. 
     
     
         18 . The method of  claim 15 , wherein the solution further comprises at least one therapeutic agent. 
     
     
         19 . The method of  claim 18 , wherein the therapeutic agent is selected from the group consisting of small molecules, peptides, proteins, and cells. 
     
     
         20 . A method of delivering a therapeutic agent to an animal in need thereof, comprising:
 administering a solution comprising the therapeutic agent to the animal, wherein the solution further comprises the peptide of  claim 1  and the solution forms a hydrogel inside the animal.   
     
     
         21 . The method of  claim 20 , wherein the animal is a mammal. 
     
     
         22 . The method of  claim 20 , wherein the animal is a human. 
     
     
         23 . The method of  claim 20 , wherein the therapeutic agent is selected from the group consisting of small molecules, peptides, proteins, and cells. 
     
     
         24 . The method of delivering a therapeutic agent to an animal in need thereof, comprising:
 administering to the animal a hydrogel comprising the therapeutic agent and the peptide of  claim 1 .   
     
     
         25 . The method of  claim 24 , wherein the animal is a mammal. 
     
     
         26 . The method of  claim 24 , wherein the animal is a human. 
     
     
         27 . The method of  claim 24 , wherein the therapeutic agent is selected from the group consisting of small molecules, peptides, proteins, and cells. 
     
     
         28 . A method of treating a wound in an animal, comprising:
 contacting the wound with a solution comprising the peptide of  claim 1 , wherein the solution forms a hydrogel.   
     
     
         29 . The method of  claim 28 , wherein the solution further comprises a therapeutic agent. 
     
     
         30 . The method of  claim 28 , wherein the animal is a mammal. 
     
     
         31 . The method of  claim 28 , wherein the animal is a human. 
     
     
         32 . The method of  claim 29 , wherein the therapeutic agent is selected from the group consisting of small molecules, peptides, proteins, and cells. 
     
     
         33 . A method of growing cells, comprising:
 forming a hydrogel comprising cells and the peptide of  claim 1 ; and   maintaining the cells under conditions suitable for cell viability.   
     
     
         34 . The method of  claim 33 , wherein forming a hydrogel comprises adjusting at least one characteristic of a solution comprising the peptide. 
     
     
         35 . The method of  claim 34 , wherein the characteristic adjusted is selected from the group consisting of pH, ionic strength, and specific ion concentration. 
     
     
         36 . The method of  claim 34 , wherein the characteristic adjusted is ionic strength. 
     
     
         37 . The method of  claim 34 , wherein the characteristic adjusted is Ca 2+  ion concentration. 
     
     
         38 . The method of  claim 33 , wherein the cells are animal cells. 
     
     
         39 . The method of  claim 33 , wherein the cells are mammalian cells. 
     
     
         40 . The method of  claim 33 , wherein the cells are human cells. 
     
     
         41 . The method of  claim 33 , wherein the cells are osteoblasts. 
     
     
         42 . The method of  claim 33 , wherein the cells are fibroblasts. 
     
     
         43 . The method of  claim 33 , wherein the cells are recombinant. 
     
     
         44 . The method of  claim 33 , wherein the cells express a protein of interest. 
     
     
         45 . The method of  claim 44 , wherein the protein of interest is an antibody. 
     
     
         46 . The method of  claim 33 , wherein the cells are stem cells. 
     
     
         47 . A method of detecting an environmental condition comprising:
 contacting a sensor comprising a hydrogel with a sample representative of the environmental condition; and   determining at least one characteristic of the hydrogel, wherein the hydrogel comprises the peptide of  claim 1 .   
     
     
         48 . The method of  claim 47 , wherein the environmental condition is the presence of an analyte of interest. 
     
     
         49 . The method of  claim 48 , wherein the characteristic altered is stiffness. 
     
     
         50 . The method of  claim 48 , wherein the characteristic altered is an optical property. 
     
     
         51 . The method of  claim 47 , further comprising comparing the characteristic of the hydrogel to the same characteristic of the hydrogel determined at a different time. 
     
     
         52 . A method of purifying a molecule of interest, comprising:
 contacting a solution comprising the molecule of interest and one or more contaminants with a hydrogel under conditions causing the molecule of interest to be retained by the hydrogel; and   recovering the molecule of interest from the hydrogel, wherein the hydrogel comprises the peptide of  claim 1 .   
     
     
         53 . The method of  claim 52 , wherein the molecule of interest is a protein. 
     
     
         54 . The method of  claim 52 , wherein the molecule of interest is an antibody. 
     
     
         55 . The method of  claim 52 , wherein the molecule of interest is a therapeutic agent. 
     
     
         56 . A method of purifying a molecule of interest, comprising:
 contacting a solution comprising the molecule of interest and one or more contaminants with a hydrogel under conditions causing at least one contaminant to be retained by the hydrogel; and   recovering the molecule of interest, wherein the hydrogel comprises the peptide of  claim 1 .   
     
     
         57 . The method of  claim 56 , wherein the molecule of interest is a protein. 
     
     
         58 . The method of  claim 56 , wherein the molecule of interest is an antibody. 
     
     
         59 . The method of  claim 56 , wherein the molecule of interest is a therapeutic agent. 
     
     
         60 . A method of encapsulating cells in a hydrogel, comprising:
 providing a solution comprising cells dispersed therein and a solution comprising the peptide of  claim 1 ; and   combining the solutions under conditions wherein a characteristic of the resulting mixture is altered such that a hydrogel is formed.   
     
     
         61 . The method of  claim 60 , wherein the characteristic altered is selected from the group consisting of pH, ionic strength, and specific ion concentration. 
     
     
         62 . The method of  claim 60 , wherein the characteristic altered is ionic strength. 
     
     
         63 . The method of  claim 60 , wherein the characteristic altered is Ca 2+  ion concentration. 
     
     
         64 . The method of  claim 60 , wherein the cells are animal cells. 
     
     
         65 . The method of  claim 60 , wherein the cells are mammalian cells. 
     
     
         66 . The method of  claim 60 , wherein the cells are human cells. 
     
     
         67 . The method of  claim 60 , wherein the cells are osteoblasts. 
     
     
         68 . The method of  claim 60 , wherein the cells are fibroblasts. 
     
     
         69 . The method of  claim 60 , wherein the cells are recombinant. 
     
     
         70 . The method of  claim 60 , wherein the cells express a protein of interest. 
     
     
         71 . The method of  claim 70 , wherein the protein of interest is an antibody. 
     
     
         72 . The method of  claim 60 , wherein the cells are stem cells. 
     
     
         73 . The method of  claim 60 , wherein the cells are human stem cells. 
     
     
         74 . The hydrogel of  claim 12 , further comprising cells, wherein the cells are evenly distributed throughout the hydrogel.