Coccidiosis vaccines

Abstract

The present invention relates to hydrophilic Eimeria polypeptides, DNA-fragments encoding those peptides, recombinant DNA molecules comprising such DNA-fragments, live recombinant carriers comprising such DNA-fragments or recombinant DNA molecules and host cells comprising such DNA-fragments, recombinant DNA molecules or live recombinant carriers. Furthermore, the invention relates to antibodies against the polypeptides and to coccidiosis vaccines based upon said polypeptides. The invention also relates to methods for the preparation of such antibodies and vaccines, and to methods for the detection of Eimeria parasites and antibodies against Eimeria parasites.

1 - 26 . (canceled) 
     
     
         27 . A DNA fragment comprising a nucleic acid sequence encoding a hydrophilic polypeptide of  Eimeria , wherein the polypeptide is an SOD-like polypeptide with a molecular weight of about 25 kD, and wherein the nucleic acid sequence comprises the sequence depicted in SEQ ID No.: 39. 
     
     
         28 . A recombinant DNA molecule comprising the DNA fragment according to  claim 27 . 
     
     
         29 . A live recombinant carrier comprising the DNA fragment according to  claim 27 . 
     
     
         30 . A host cell comprising the DNA fragment according to  claim 27 . 
     
     
         31 . A method for detecting  Eimeria  parasites in poultry, comprising isolating a DNA preparation from the poultry to be tested, incubating the DNA preparation with DNA fragments according to  claim 27 , and detecting hybridization of the DNA fragments with DNA from the DNA preparation. 
     
     
         32 . A DNA fragment comprising a nucleic acid sequence encoding a hydrophilic polypeptide of  Eimeria , wherein the polypeptide is a peroxidoxin-like polypeptide with a molecular weight of about 25 kD, and wherein the nucleic acid sequence comprises the sequence depicted in SEQ ID NO.: 40. 
     
     
         33 . A recombinant DNA molecule comprising the DNA fragment according to  claim 32 . 
     
     
         34 . A live recombinant carrier comprising the DNA fragment according to  claim 32 . 
     
     
         35 . A host cell comprising the DNA fragment according to  claim 32 . 
     
     
         36 . A method for detecting  Eimeria  parasites in poultry, comprising isolating a DNA preparation from the poultry to be tested, incubating the DNA preparation with DNA fragments according to  claim 32 , and detecting hybridization of the DNA fragments with DNA from the DNA preparation. 
     
     
         37 . A DNA fragment comprising a nucleic acid sequence encoding a hydrophilic polypeptide of  Eimeria , wherein the polypeptide is a peroxidoxin-like polypeptide, and wherein the nucleic acid sequence comprises the sequence depicted in SEQ ID NO.: 41. 
     
     
         38 . A recombinant DNA molecule comprising the DNA fragment according to  claim 37 . 
     
     
         39 . A live recombinant carrier comprising the DNA fragment according to  claim 37 . 
     
     
         40 . A host cell comprising the DNA fragment according to  claim 37 . 
     
     
         41 . A method for detecting  Eimeria  parasites in poultry, comprising isolating a DNA preparation from the poultry to be tested, incubating the DNA preparation with DNA fragments according to  claim 37 , and detecting hybridization of the DNA fragments with DNA from the DNA preparation.