Tissue regeneration

Abstract

A method including positioning a delivery device at a location in a vessel within a mammalian body, introducing a first treatment agent including a cellular component through the delivery device, and introducing a different second treatment agent disposed in a carrier through the delivery device. A method including identifying an infarct region within myocardial tissue and a border region of perfused tissue adjacent the infarct region, introducing a treatment agent including a cellular component to the border region, and introducing a plurality of microparticles to the infarct region. A kit including a treatment agent including a cellular component in a form suitable for percutaneous delivery, and a separate amount of a plurality of microparticles in a form suitable for percutaneous delivery.

1 . A kit comprising:
 a treatment agent comprising a celluar component in a form suitable for percutaneous delivery; and   a separate amount of a plurality of microparticles in a form suitable for percutaneous delivery.   
     
     
         2 . The kit of  claim 1 , wherein the plurality of microparticles comprise a non-immunogenic collagen. 
     
     
         3 . The kit of  claim 1 , wherein the plurality of microparticles comprises a hydrogel. 
     
     
         4 . The kit of  claim 3 , wherein the hydrogel comprises a modified alginate. 
     
     
         5 . The kit of  claim 1 , wherein the plurality of microparticles comprises an encapsulation polymer. 
     
     
         6 . The kit of  claim 1 , wherein each of the plurality of microparticles comprise a release rate of dispensing a second treatment agent and collectively the plurality of microparticles comprise a release rate gradient. 
     
     
         7 . The kit of  claim 1 , wherein a portion of the plurality of microparticles comprise a treatment agent that affects therapeutic angiogenesis. 
     
     
         8 . The kit of  claim 7 , wherein the treatment agent comprises a growth factor. 
     
     
         9 . The kit of  claim 1 , wherein a portion of the plurality of microparticles comprise an auxiliary agent having a property auxiliary to affecting therapeutic angiogenesis. 
     
     
         10 . The kit of  claim 9 , wherein the auxiliary agent comprises a homing factor.