Fucose transporter

Abstract

The present invention provides a gene encoding a fucose transporter, a fucose transporter polypeptide, a method for screening for a compound that binds to a fucose transporter or a compound that inhibits fucose transport activity, a cell having inhibited fucose transporter functions, and a cell wherein the expression of the fucose transporter is inhibited. The present invention further relates to a method for producing recombinant protein, and specifically, to a method for producing protein by which fucose existing within the Golgi apparatus of a host cell is decreased, a method for inhibiting the addition of fucose to protein by which fucose existing within the Golgi apparatus of a host cell is decreased upon production of recombinant protein using the host cell, a method for increasing the cytotoxic activity of an antibody by which an antibody is produced using a cell wherein fucose existing within the Golgi apparatus is decreased, and a cell having a Golgi apparatus wherein fucose existing within the Golgi apparatus is decreased.

1 . A method for producing a recombinant protein, wherein fucose existing in the Golgi apparatus of a host cell is decreased. 
     
     
         2 . The method for producing a recombinant protein according to  claim 1 , wherein the fucose transporter functions are inhibited by artificially suppressing the expression of the fucose transporter in a host cell. 
     
     
         3 . The method for producing a recombinant protein according to  claim 2 , wherein the expression of the fucose transporter is suppressed using RNAi. 
     
     
         4 . The method for producing a recombinant protein according to  claim 1 , wherein the fucose transporter functions are inhibited by deleting a gene encoding the fucose transporter in a host cell. 
     
     
         5 . The method for producing a recombinant protein according to  claim 1 , wherein the protein is an antibody. 
     
     
         6 . The method for producing a recombinant protein according to  claim 1 , wherein a protein not having fucose added thereto is produced. 
     
     
         7 . The method for producing a recombinant protein according to  claim 1 , wherein the host cell is a CHO cell.