Matrix and Dynamic Polymer Systems and Compositions for Microchannel Separation

Abstract

Matrix polymers and dynamic coating polymers, compositions thereof and related methods, systems and apparatus for microchannel separation.

1 . A DNA sequencing or genotyping composition for microchannel electrophoresis comprising a hydrophobic separation matrix component and a hydrophilic wall coating component wherein said microchannel electrophoresis sequences of from between 500 to 800 bases in 12 minutes or less. 
     
     
         2 . The composition of  claim 1  wherein said hydrophobic separation matrix component comprises a polyacrylamide of a formula CH 2 C(R)C(O)NR′R″ n , wherein R is selected from H and methyl, and R′ and R″ are independently selected from C 1  to about C 8  linear alkyl moieties C 1  to about C 8  alkoxy-substituted linear alkyl moieties, C 1  to about C 8  branched alkyl moieties and C 1  to about C 8  alkoxy-substituted branched alkyl moieties, and copolymers thereof, said polyacrylamide at least partially water soluble; and said hydrophilic wall coating component comprising a poly(N-hydroxyethylacrylamide). 
     
     
         3 . The composition of  claim 2 , wherein said matrix component is selected from poly(N,N-dimethylacrylamide) and a poly(N,N-dimethylacrylamide) copolymer. 
     
     
         4 . The composition of  claim 3 , wherein said matrix component comprises about 3% (w/v) to about 5% (w/v) poly(N,N-dimethylacrylamide) in an aqueous medium. 
     
     
         5 . The composition of  claim 4 , wherein said matrix component comprises about 3% (w/v) poly(N,N-dimethylacrylamide), with a weight average molar mass ranging from about 3 to about 5 MDa. 
     
     
         6 . The composition of  claim 4 , wherein said matrix component comprises about 1% (w/v) to about 2% (w/v) poly(N,N-dimethylacrylamide), with a weight average molar mass ranging from about 200 to about 300 kDa. 
     
     
         7 . The composition of  claim 1  wherein said microchannel electrophoresis sequences of from between 500 to 600 bases in less than 7 minutes. 
     
     
         8 . The composition of  claim 1  wherein said microchannel electrophoresis sequences up to 630 bases in about 9 minutes. 
     
     
         9 . The composition of  claim 1  wherein said microchannel electrophoresis sequences about 800 bases in about 12 minutes. 
     
     
         10 . A microchannel electrophoresis system for DNA and RNA separations, said system comprising a hydrophobic separation matrix component and a hydrophilic wall coating component wherein said system sequences of from between 500 to 800 bases in 12 minutes or less. 
     
     
         11 . The system of  claim 10  wherein said hydrophobic separation matrix component comprises a poly(N,N-dimethylacrylamide) and said hydrophilic wall coating component comprises a poly(N-hydroxyethylacrylamide). 
     
     
         12 . The system of  claim 11  further comprising a microchannel substrate selected from a micro-dimensioned capillary, said capillary defining an internal dimension ranging from about 10 microns to about 150 microns, and a microfluidic electrophoresis chip comprising a microchannel ranging from about 10 microns to about 150 microns. 
     
     
         13 . The system of  claim 12 , wherein said matrix component comprises about 3% (w/v) poly(N,N-dimethylacrylamide), with a weight average molar mass ranging from about 3 to about 5 MDa, and about 1% (w/v) to about 2% (w/v) poly(N,N-dimethylacrylamide), with a weight average molar mass ranging from about 200 to about 300 kDa. 
     
     
         14 . The system of  claim 13 , wherein said poly(N-hydroxyethylacrylamide) is contacted with said substrate. 
     
     
         15 . The system of  claim 10  wherein said system sequences of from between 500 to 600 bases in less than 7 minutes. 
     
     
         16 . The system of  claim 10  wherein said system sequences up to 630 bases in about 9 minutes. 
     
     
         17 . The system of  claim 10  wherein said system sequences about 800 bases in about 12 minutes.