Isolated pediococcus acidilactici 05b0111 and method of producing exopolysaccharide

Abstract

Disclosed herein is an isolated Pediococcus acidilactici 05B0111 capable of producing an exopolysaccharide. The Pediococcus acidilactici 05B0111 is deposited in Biosource Collection and Research Center (BCRC) of Food Industry Research and Development Institute (FIRDI) under an accession number BCRC 910420 and deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) under an accession number DSM 22345. A method of producing an exopolysaccharide is also disclosed. The method involves cultivating an isolated Pediococcus acidilactici in a suitable medium under condition such that the exopolysaccharide is formed. A pharmaceutical composition including the aforementioned Pediococcus acidilactici 05B0111 and a food product including the aforementioned Pediococcus acidilactici 05B0111 are disclosed as well.

1 . An isolated  Pediococcus acidilactici  05B0111 capable of producing an exopolysaccharide, said  Pediococcus acidilactici  05B0111 being deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) under an accession number DSM 22345. 
     
     
         2 . A method of producing an exopolysaccharide comprising cultivating an isolated  Pediococcus acidilactici  in a suitable medium under condition such that the exopolysaccharide is formed. 
     
     
         3 . The method of  claim 2 , wherein the isolated  Pediococcus acidilactici  is an isolated  Pediococcus acidilactici  05B0111 capable of producing an exopolysaccharide, said  Pediococcus acidilactici  05B0111 being deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) under an accession number DSM 22345. 
     
     
         4 . The method of  claim 2 , further comprising purifying the exopolysaccharide. 
     
     
         5 . The method of  claim 2 , wherein the suitable medium comprises a carbon source selected from the group consisting of: lactose, fructose, maltose, glucose, molasses, galactose, xylose, xylitol, inulin, sorbitol, trehalose, sucrose, and mixtures thereof. 
     
     
         6 . The method of  claim 5 , wherein the suitable medium is an edible material. 
     
     
         7 . The method of  claim 6 , wherein the edible material is selected from the group consisting of: a fluid milk product, milk powder, fruit juice, soybean milk, vegetable-fruit juice, health food, animal feed, an agricultural product, a livestock product, an aquatic product, and a functional ingredient. 
     
     
         8 . The method of  claim 2 , wherein, before cultivating, the suitable medium has a pH value ranging from 3 to 7. 
     
     
         9 . The method of  claim 8 , wherein, before cultivating, the suitable medium has a pH value of 5. 
     
     
         10 . The method of  claim 2 , wherein the cultivating step is conducted at a temperature ranging from 25° C. to 37° C. 
     
     
         11 . A pharmaceutical composition comprising an isolated  Pediococcus acidilactici  05B0111 of  claim 1 . 
     
     
         12 . The pharmaceutical composition of  claim 11 , further comprising an exopolysaccharide obtained by cultivating an isolated  Pediococcus acidilactici  in a suitable medium under condition such that the exopolysaccharide is formed, wherein the isolated  Pediococcus acidilactici  is an isolated  Pediococcus acidilactici  05B0111 capable of producing an exopolysaccharide, said  Pediococcus acidilactici  05B0111 being deposited in Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ) under an accession number DSM 22345. 
     
     
         13 . The pharmaceutical composition of  claim 11 , further comprising a pharmaceutically acceptable carrier. 
     
     
         14 . A food product comprising an isolated  Pediococcus acidilactici  05B0111 of  claim 1 . 
     
     
         15 . The food product of  claim 14 , further comprising an exopolysaccharide obtained by cultivating an isolated  Pediococcus acidilactici  in a suitable medium under condition such that the exopolysaccharide is formed, wherein the isolated  Pediococcus acidilactici  is an isolated  Pediococcus acidilactici  05B0111 capable of producing an exopolysaccharide, said  Pediococcus acidilactici  05B0111 being deposited in Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ) under an accession number DSM 22345.