Method for quantification of soluble lr11

Abstract

Provided is a method for quantifying soluble LR11 in a biological sample such as serum by an immunological means conveniently and accurately without the need of carrying out any complicated separation manipulation. An immunological quantification method for soluble LR11 in a sample derived from a mammal, including a step of treating the sample with at least one surfactant selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyalkylene alkyl phenyl ether, an alkyl glycoside, an alkylthio glycoside, an acyl-N-methylglucamide and a salt of cholic acid.

1 . An immunological method for quantifying soluble LR11 in a sample derived from a mammal, comprising a step of treating the sample with at least one surfactant selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyalkylene alkyl phenyl ether, an alkyl glycoside, an alkyl thioglycoside, an acyl-N-methylglucamine and a salt of cholic acid. 
     
     
         2 . The immunological method for quantifying according to  claim 1 , wherein the sample is blood, plasma, serum, cerebrospinal fluid or urine. 
     
     
         3 . The immunological method for quantifying according to  claim 1  or  2 , wherein the immunological method for quantifying further comprises a step of forming an immunocomplex by using at least two kinds of antibodies each having an antigen recognition site which is different from each other. 
     
     
         4 . The immunological method for quantifying according to any one of  claims 1  to  3 , wherein the surfactant is acyl-N-methylglucamine. 
     
     
         5 . A kit for immunological quantification of soluble LR11, comprising an anti-soluble LR11 antibody and at least one surfactant selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyalkylene alkyl phenyl ether, an alkyl glycoside, an alkyl thioglycoside, an acyl-N-methylglucamine and a salt of cholic acid. 
     
     
         6 . A use of a combination comprising an anti-soluble LR11 antibody and at least one surfactant selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyalkylene alkyl phenyl ether, an alkyl glycoside, an alkyl thioglycoside, an acyl-N-methylglucamine and a salt of cholic acid for manufacturing a kit for immunological quantification of soluble LR11.