Anti-aging or antioxidant composition containing plant stem cell line derived from cambium of panax ginseng

Abstract

The present invention relates to an anti-aging or antioxidant composition which contains, as an active ingredient, a plant stem cell line derived from the cambium of Panax ginseng, including wild ginseng and ginseng, or an extract thereof, a lysate thereof and a culture thereof. The composition has minimized side effects compared to existing anti-aging agents and antioxidants, and thus is safe for the skin. Also, the composition of the present invention shows an antioxidant effect of inhibiting reactive oxygen species caused by exposure to UV radiation that is the major cause of skin aging, and it can effectively reduce or inhibit aging-related factors. Thus, the composition is useful for the prevention and inhibition of aging.

1 . An anti-aging or antioxidant composition containing any one or more of a cell line, which is derived from a cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof:
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by a number of vacuoles.   
     
     
         2 . The anti-aging or antioxidant composition according to  claim 1 , wherein the cell line is additionally characterized in that:
 (a) it is present as single cells during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng;  and   (c) it has a higher growth rate than those of cell lines derived from tissues other than the cambium of  Panax ginseng  and is cultured stably.   
     
     
         3 . The anti-aging or antioxidant composition according to  claim 1 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a storage root tissue containing the cambium of  Panax ginseng;      (b) applying osmotic stress to the obtained cambium-containing storage root tissue, and then culturing the cambium-containing storage root tissue in a IAA (Indole-3-acetic acid)- or IBA (Indole-3-butyric acid)-containing medium, thereby inducing a cambium-derived cell line; and   (c) collecting the induced cambium-derived cell line.   
     
     
         4 . The anti-aging or antioxidant composition according to  claim 1 , wherein the culture is obtained by additionally culturing the cell line in a medium which contains 3-5 wt % of raw sugar or sugar; or at least one substance selected from the group consisting of methyl jasmonate, fungal extract, bacterial extract, yeast extract, chitosan, glucomannan, glucan, phenylalanine, benzoic acid, salicylic acid, arachidonic acid, STS, mevalonalonate N-benzolyglycine, ABA, SNP, IPP, BHT, CCC, ethephon, hippuric acid, ammonium ceric nitrate, AgNO 3 , vanadyl sulfate, p-aminobenzoic acid, brassinosteroids, sodium alginate, and sodium acetate. 
     
     
         5 . The anti-aging or antioxidant composition according to any one among  claims 1  to  3 , wherein the  Panax ginseng  is wild ginseng or ginseng. 
     
     
         6 . The anti-aging or antioxidant composition according to  claim 1 , wherein the extract is obtained using a solvent selected from the group consisting of distilled water, alcohol, acetone, DMSO (dimethyl sulfoxide), and mixed solvents thereof. 
     
     
         7 . The anti-aging or antioxidant composition according to  claim 1 , wherein the extract is obtained by fractionating the cell line sequentially with distilled water, methanol and acetone. 
     
     
         8 . An anti-aging cosmetic composition containing any one or more of a cell line, which is derived from a cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof:
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by a number of vacuoles.   
     
     
         9 . The anti-aging cosmetic composition according to  claim 8 , wherein the cell line is wherein the cell line is additionally characterized in that:
 (a) it is present as single cells during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng;  and   (c) it has a higher growth rate than those of cell lines derived from tissues other than the cambium of  Panax ginseng  and is cultured stably.   
     
     
         10 . The anti-aging cosmetic composition according to  claim 8 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a storage root tissue containing the cambium of  Panax ginseng;      (b) applying osmotic stress to the obtained cambium-containing storage root tissue, and then culturing the cambium-containing storage root tissue in a IAA (Indole-3-acetic acid)- or IBA (Indole-3-butyric acid)-containing medium, thereby inducing a cambium-derived cell line; and   (c) collecting the induced cambium-derived cell line.   
     
     
         11 . The anti-aging cosmetic composition according to  claim 8 , wherein the culture is obtained by additionally culturing the cell line in a medium which contains 3-5 wt % of raw sugar or sugar; or at least one substance selected from the group consisting of methyl jasmonate, fungal extract, bacterial extract, yeast extract, chitosan, glucomannan, glucan, phenylalanine, benzoic acid, salicylic acid, arachidonic acid, STS, mevalonalonate N-benzolyglycine, ABA, SNP, IPP, BHT, CCC, ethephon, hippuric acid, ammonium ceric nitrate, AgNO 3 , vanadyl sulfate, p-aminobenzoic acid, brassinosteroids, sodium alginate, and sodium acetate. 
     
     
         12 . The anti-aging cosmetic composition according to any one among  claims 8  to  11 , wherein the composition inhibits MMP-1 production. 
     
     
         13 . An anti-aging or antioxidant functional food containing any one or more of a cell line, which is derived from a cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof:
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by a number of vacuoles.   
     
     
         14 . The anti-aging or antioxidant functional food according to  claim 13 , wherein the cell line is additionally characterized in that:
 (a) it is present as single cells during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng;  and   (c) it has a higher growth rate than those of cell lines derived from tissues other than the cambium of  Panax ginseng  and is cultured stably.   
     
     
         15 . The anti-aging or antioxidant functional food according to  claim 13 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a storage root tissue containing the cambium of  Panax ginseng;      (b) applying osmotic stress to the obtained cambium-containing storage root tissue, and then culturing the cambium-containing storage root tissue in a IAA (Indole-3-acetic acid)- or IBA (Indole-3-butyric acid)-containing medium, thereby inducing a cambium-derived cell line; and   (c) collecting the induced cambium-derived cell line.   
     
     
         16 . The anti-aging or antioxidant functional food according to  claim 13 , wherein the culture is obtained by additionally culturing the cell line in a medium which contains 3-5 wt % of raw sugar or sugar; or at least one substance selected from the group consisting of methyl jasmonate, fungal extract, bacterial extract, yeast extract, chitosan, glucomannan, glucan, phenylalanine, benzoic acid, salicylic acid, arachidonic acid, STS, mevalonalonate N-benzolyglycine, ABA, SNP, IPP, BHT, CCC, ethephon, hippuric acid, ammonium ceric nitrate, AgNO 3 , vanadyl sulfate, p-aminobenzoic acid, brassinosteroids, sodium alginate, and sodium acetate.