Antibodies for inhibiting blood coagulation and methods of use thereof

Abstract

Disclosed are antibodies that provide superior anti-coagulant activity by binding native human TF with high affinity and specificity. Also disclosed are methods of using such antibodies to reduce cancer cell tissue factor activity and to detect cancer cells that express TF.

1 - 33 . (canceled) 
     
     
         34 . A method of reducing tissue factor levels in a mammal comprising: administering to the mammal a therapeutically effective amount of an antibody capable of binding native tissue factor, the antibody linked covalently to a cell toxin or an effector molecule to provide complement-fixing ability and antibody-dependent cell-mediated cytotoxicity, the antibody contacting cells expressing tissue factor to reduce tissue factor levels in the mammal. 
     
     
         35 . The method of  claim 34  wherein the cells expressing tissue factor are cancer cells, immune cells, or endothelial cells. 
     
     
         36 . A method of detecting tissue factor in a biological sample comprising: contacting a biological sample with a monoclonal antibody of claim  1  and analyzing the biological sample and monoclonal antibody for the presence of tissue factor in the biological sample.