Pharmaceutical composition for preventing and treating cell proliferative disease comprising mixture of feather of birds and scale of fish as an active ingredient

Abstract

The present invention relates to a pharmaceutical composition for preventing and treating cell proliferative diseases comprising a feather of birds and a scale of fish, a scale transformed from the dermis, a degenerated or cornified variant of a scale, or a scale or horny scale of reptiles as an active ingredient. More particularly, the present invention relates to a pharmaceutical composition for preventing and treating cell proliferative diseases comprising a mixture of 70-85 weight % of a feather of birds and 15-30 weight % of a scale of fish, a scale transformed from the dermis, a degenerated or cornified variant of a scale, or a scale or horny scale of reptiles as an active ingredient. The inventive composition has the effect of inhibiting and preventing growth of cancer cells. Accordingly, the inventive composition may be used for anticancer purposes to prevent, ameliorate or treat cancer.

1 . A pharmaceutical composition for preventing and treating cell proliferative diseases comprising 70˜85 weight % of a feather of birds and 15˜30 weight % of a scale of fish as an active ingredient. 
     
     
         2 . A pharmaceutical composition for preventing and treating cell proliferative diseases comprising 70˜85 weight % of a feather of birds and 15˜30 weight % of a scale of fish, a scale transformed from the dermis, a degenerated or cornified variant of a scale, or a scale or horny scale of reptiles as an active ingredient. 
     
     
         3 . The composition of  claim 1  or  claim 2 , wherein the feather of birds is a powder of a whole feather, a calamus or a rachis. 
     
     
         4 . The composition of  claim 3 , wherein a powder of a calamus or a rachis is prepared by a method comprising the steps of:
 (a) immersing a feather of birds in water of 20° C.˜77° C. for 1 minute to 2 hours.   (b) sterilizing the immersed feather in brine with a salinity from 4.0 to 6.0 for 10 minutes to 2 hours.   (c) washing the feather with flowing water to remove impurities.   (d) immersing and sterilizing the feather in 0.1˜0.2 weight % (v/v) sodium chloride (NaCl) solution for 5 minutes to 1 hour   (e) drying the feather at 15° C.˜30° C. for 30 minutes to 72 hours.   (f) sterilizing the dried feather by irradiating 10˜400 nm UV for 10 minutes to 2 hours.   (g) separating a calamus or a rachis from the sterilized feather.   (h) sterilizing the separated calamus or rachis by irradiating 10˜400 nm UV for 10 minutes to 2 hours.   (i) cutting the sterilized calamus or rachis to 2˜7 mm for easier grinding.   (j) sterilizing the cut feather by irradiating 10˜400 nm UV for 10 minutes to 2 hours.   (k) grinding the cut and sterilized calamus or rachis   (l) sterilizing the resulting powder by irradiating 10˜400 nm UV for 10 minutes to 2 hours.   
     
     
         5 . The position of  claim 1  or  claim 2 , wherein the birds are selected from the group consisting of chicken, duck, turkey, goose and ostrich. 
     
     
         6 . The position of  claim 1 , wherein a scale of fish is prepared by a method comprising the steps of:
 (a) immersing a fish in water of 20° C.˜77° C. for 1 minute to 2 hours, and separating and washing a scale of the fish   (b) immersing and sterilizing the scale washed in the step (a) in 0.1˜0.2 weight % (w/v) NaCl solution for 5 minutes to 1 hour.   (c) immersing and sterilizing the scale immersed in the step (b) in 1˜3 weight % (v/v) vinegar solution for 15˜20 minutes.   (d) drying the scale immersed in the step (c) at 30° C.˜70° C. for 5 minutes to 48 hours.   (e) sterilizing the scale dried in the step (d) by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   (f) cutting the scale sterilized in the step (e) to 2˜7 mm for easier grinding.   (g) sterilizing the scale cut in the step (f) by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   (h) grinding the scale sterilized in the step (g) using a grinding machine.   (i) sterilizing the powder resulting from the step (h) by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   
     
     
         7 . The position of  claim 1 , wherein the fish is selected from the group consisting of gray mullet, perch and rock trout, carp, crucian carp and trout. 
     
     
         8 . The position of  claim 1  or  claim 2 , wherein cell proliferative diseases are neoplastic diseases caused by tumor. 
     
     
         9 . The position of  claim 8 , wherein the neoplastic diseases are cancer. 
     
     
         10 . The position of  claim 9 , wherein the cancer is selected from the group consisting of large intestine cancer, spleen cancer, colon cancer, lung cancer, liver cancer, stomach cancer, esophageal cancer, pancreatic cancer, gallbladder cancer, kidney cancer, bladder cancer, prostate cancer, testicular cancer, cervical cancer, endometrial cancer, choriocarcinoma, ovarian cancer, breast cancer, thyroid cancer, brain cancer, head and neck cancer, malignant melanoma, lymphoma, bone cancer, soft tissue sarcoma, spinal cancer. 
     
     
         11 . The composition of  claim 2 , wherein the scale transformed from the dermis, the degradated or cornified variant of a scale, or the scale or horny scale of reptiles is prepared by a method comprising the steps of:
 (a) applying Physical force to a dead body of fish or reptiles so as to separate the scale or skin;   (b) sterilizing the separated scale or skin in brine with a salinity from 4.0 to 6.0 for 10 minutes to 2 hours;   (c) immersing the scale or skin in 1˜3% (v/v) vinegar solution for 10˜20 minutes and sterilizing;   (d) washing the scale or skin with flowing water to remove impurities;   (e) drying the washed scale or skin at 20° C.˜70° C. for 5 minutes to 72 hours;   (f) sterilizing the dried scale or skin by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (g) cutting the scale or skin to 3˜4 mm in width and length;   (h) sterilizing the cut scale or skin by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (i) grinding the scale or skin using a grinding machine; and   (j) sterilizing the resulting powder by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   
     
     
         12 . The composition of  claim 2 , wherein the reptile is a snake. 
     
     
         13 . A method for preparing a powder of a calamus or a rachis comprising the steps of:
 (a) immersing a feather of birds in water of 20° C.˜77° C. for 1 minute to 2 hours;   (b) sterilizing the immersed feather in brine with a salinity from 4.0 to 6.0 for 10 minutes to 2 hours;   (c) washing the feather well with flowing water to remove impurities;   (d) immersing the feather in 0.1˜0.2 weight % (v/v) NaCl solution for 5 minutes to 1 hour and sterilizing the same;   (e) drying the feather at 15° C.˜30° C. for 30 minutes to 72 hours;   (f) sterilizing the dried feather by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (g) separating a calamus or a rachis from the sterilized feather;   (h) sterilizing the separated calamus or rachis by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (i) cutting the sterilized calamus or rachis to 2˜7 mm for easier grinding;   (j) sterilizing the cut feather by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (k) grinding the cut and sterilized calamus or rachis; and   (l) sterilizing the resulting powder by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   
     
     
         14 . A method for preparing a powder of a scale of fish comprising the steps of:
 (a) immersing a fish in water of 20° C.˜77° C. for 1 minute to 2 hours and separating and washing a scale of the fish;   (b) immersing and sterilizing the scale washed in the step (a) in 0.1˜0.2 weight % (w/v) NaCl solution for 5 minutes to 1 hour;   (c) immersing and sterilizing the scale immersed in the step (b) in 1˜3 weight % (v/v) vinegar solution for 15˜20 minutes;   (d) drying the scale immersed in the step (c) at 30° C.˜70° C. for 5 minutes to 48 hours;   (e) sterilizing the scale dried in the step (d) by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (f) cutting the scale sterilized in the step (e) to 2˜7 mm for easier grinding;   (g) sterilizing the scale cut in the step (f) by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (h) grinding the scale sterilized in the step (g) using a grinding machine; and   (i) sterilizing the powder resulting from the step (h) by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   
     
     
         15 . A method for preparing a powder of a scale transformed from the dermis, the degenerated or cornified variant of a scale, or the scale or horny scale of reptiles comprising the steps of:
 (a) separating a scale transformed from the dermis, the degraded or cornified variant of a scale, or the scale or horny scale of reptiles from a dead body by applying physical force;   (b) sterilizing the separated scale or skin in brine with a salinity from 4.0 to 6.0 for 10 minutes to 2 hours;   (c) immersing and sterilizing the scale or skin in 1˜3% (v/v) vinegar solution for 10˜20 minutes;   (d) washing the scale or skin with flowing water to remove impurities;   (e) drying the washed scale or skin at 20° C.˜70° C. for 5 minutes to 72 hours;   (f) sterilizing the dried scale or skin by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (g) cutting the scale or skin to 3˜4 mm in width and length;   (h) sterilizing the cut scale or skin by irradiating with 10˜400 nm UV for 10 minutes to 2 hours;   (i) grinding the scale or skin using a grinding machine; and   (j) sterilizing the resulting powder by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.   
     
     
         16 . An administration method of a composition of  claim 1  or  claim 2 , wherein it is administered orally. 
     
     
         17 . The method of anyone of  claims 13  to  15 , wherein the method comprises further steps of sterilizing using brine with a salinity from 4.0 to 6.0 and 3% (v/v) vinegar solution; and sterilizing by irradiating with 10˜400 nm UV for 10 minutes to 2 hours.