Telomelysin/GFP-expressing recombinant virus

Abstract

The present invention provides a reagent for cancer cell detection or cancer diagnosis. The present invention relates to a reagent for cancer cell detection, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome.

1 - 19 . (canceled) 
     
     
         20 . A reagent for cancer cell detection, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome. 
     
     
         21 . A reagent for cancer diagnosis, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein, and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome. 
     
     
         22 . The reagent according to  claim 20  or  21 , wherein the reagent is used for in vivo cancer detection or diagnosis, or for navigation surgery. 
     
     
         23 . The reagent according to  claim 20  or  21 , wherein the promoter from human telomerase is hTERT promoter. 
     
     
         24 . The reagent according to  claim 20  or  21 , wherein the labeling protein is a fluorescent protein, 
     
     
         25 . The reagent according to  claim 24 , wherein the fluorescent protein is selected from the group consisting of green fluorescent protein (GFP), enhanced-humanized GFP (EGFP), red-shift GFP (rsGFP), yellow fluorescent protein (YFP), cyan fluorescent protein (CFP), blue fluorescent protein (BFP) and  Renilla reniformis -derived GFP. 
     
     
         26 . The reagent according to  claim 24 , wherein the fluorescent protein is GFP or EGFP. 
     
     
         27 . The reagent according to  claim 20  or  21 , wherein the promoter capable of regulating the expression of the gene encoding a labeling protein is a cytomegalovirus promoter. 
     
     
         28 . The reagent according to claim  1  or  2 , wherein the virus is an adenovirus.