Composition Comprising Bamboo Extract and the Compounds Isolated Therefrom Showing Treating and Preventing Activity for Inflammatory and Blood Circulation Disease

Abstract

The present invention relates to a composition comprising a bamboo plant extract for the prevention and treatment of inflammatory or blood circulation disease. The extracts from bamboo plant have potent anti-inflammatory activity by inhibiting NO production and circulation-improving activity by inhibiting elastase activity and, healing the wound of vascular endothelial cell, activating u-PA expression and inhibiting PAI-1 expression, lowering cholesterol deposit and inhibiting neointima formation, therefore it can be used as the therapeutics or health care food for treating and preventing inflammatory or blood circulation diseases.

1 . A method for the prevention and treatment of cardiovascular disease comprising administering to a subject in need thereof an effective amount of a composition comprising the crude extract or non-polar solvent soluble extract of a Bamboo plant. 
     
     
         2 . The method according to  claim 1  wherein said crude extract is extracted with the solvent selected from the group consisting of water, lower alcohol and the mixture thereof. 
     
     
         3 . The method according to  claim 1  wherein said non-polar solvent soluble extract is extracted with non-polar solvent selected from the group consisting of hexane, ethyl acetate, chloroform and dichloromethane. 
     
     
         4 . The method according to  claim 1  wherein said bamboo plant is selected from the  Sasa  genus or the  Phyllostachys  genus. 
     
     
         5 . The method according to  claim 4  wherein said bamboo selected from the group consisting of  Sasa borealis  Makino,  Sasa coreana  Nakai,  Sasa japonica  Makino,  Sasa borealis  var.  gracilis  and  Sasa palmata  Nakai. 
     
     
         6 . The method according to  claim 4  wherein said bamboo plant selected from the group consisting of  Phyllostachys bambusoides  SIEB. Et Zucc,  Phyllostachys nigra  MUNRO,  Phyllostachys nigra  MUNRO var.  henonis  STAPF and  Phyllostachys pubescens  MAZEL ex H. de LEH. 
     
     
         7 . The method according to  claim 1  wherein said cardiovascular disease is selected from the group consisting of hypertension, heart disease, brain stroke, peripheral blood disease, stenosis, or cardiac infarction. 
     
     
         8 . A method for the prevention and treatment of blood circulation disease comprising administering to a subject in need thereof an effective amount of a composition comprising the crude extract or non-polar solvent soluble extract of Bamboo plant. 
     
     
         9 . The method according to  claim 8  wherein said crude extract is extracted with the solvent selected from the group consisting of water, lower alcohol and the mixture thereof. 
     
     
         10 . The method according to  claim 8  wherein said non-polar solvent soluble extract is extracted with non-polar solvent selected from the group consisting of hexane, ethyl acetate, chloroform and dichloromethane. 
     
     
         11 . The method according to  claim 8  wherein said bamboo is selected from the  Sasa  genus or the  Phyllostachys  genus. 
     
     
         12 . The method according to  claim 11  wherein said bamboo selected from the group consisting of  Sasa borealis  Makino,  Sasa coreana  Nakai,  Sasa japonica  Makino,  Sasa borealis  var.  gracilis  and  Sasa palmata  Nakai. 
     
     
         13 . The method according to  claim 11  wherein said bamboo plant selected from the group consisting of  Phyllostachys bambusoides  SIEB. Et Zucc,  Phyllostachys nigra  MUNRO,  Phyllostachys nigra  MUNRO var.  henonis  STAPF and  Phyllostachys pubescens  MAZEL ex H. de LEH. 
     
     
         14 . A method for the prevention and treatment of inflammatory disease comprising administering to a subject in need thereof an effective amount of a composition comprising the crude extract or non-polar solvent soluble extract of Bamboo plant. 
     
     
         15 . The method according to  claim 14  wherein said crude extract is extracted with the solvent selected from the group consisting of water, lower alcohol and the mixture thereof. 
     
     
         16 . The method according to  claim 14  wherein said non-polar solvent soluble extract is extracted with non-polar solvent selected from the group consisting of hexane, ethyl acetate, chloroform and dichloromethane. 
     
     
         17 . The method according to  claim 14  wherein said bamboo is selected from the  Sasa  genus or the  Phyllostachys  genus. 
     
     
         18 . The method according to  claim 17  wherein said bamboo selected from the group consisting of  Sasa borealis  Makino,  Sasa coreana  Nakai,  Sasa japonica  Makino,  Sasa borealis  var.  gracilis  and  Sasa palmata  Nakai. 
     
     
         19 . The method according to  claim 17  wherein said bamboo plant selected from the group consisting of  Phyllostachys bambusoides  SIEB. Et Zucc,  Phyllostachys nigra  MUNRO,  Phyllostachys nigra  MUNRO var.  henonis  STAPF and  Phyllostachys pubescens  MAZEL ex H. de LEH. 
     
     
         20 . The method according to  claim 14  wherein said inflammatory disease is selected from the group consisting of arthritis, atherosclerosis, gastritis, colitis, nephritis, hepatitis, cancer or degenerative disease. 
     
     
         21 . A method for treating a symptom of a blood circulation disorder wherein said symptom is selected from the group consisting of benumbed feet or hands, crick of neck or shoulders, loss of memory, lethargy, loss of concentration, vertigo and chronic fatigue said method comprising administering to a subject in need thereof an effective amount of a composition comprising the crude extract or non-polar solvent soluble extract of Bamboo plant. 
     
     
         22 . The method according to  claim 21  wherein said crude extract is extracted with the solvent selected from the group consisting of water, lower alcohol and the mixture thereof. 
     
     
         23 . The method according to  claim 21  wherein said non-polar solvent soluble extract is extracted with non-polar solvent selected from the group consisting of hexane, ethyl acetate, chloroform and dichloromethane. 
     
     
         24 . The method according to  claim 21  wherein said bamboo is selected from the  Sasa  genus or the  Phyllostachys  genus. 
     
     
         25 . The method according to  claim 24  wherein said bamboo selected from the group consisting of  Sasa borealis  Makino,  Sasa coreana  Nakai,  Sasa japonica  Makino,  Sasa borealis  var.  gracilis  and  Sasa palmata  Nakai. 
     
     
         26 . The method according to  claim 25  wherein said bamboo plant is selected from the group consisting of  Phyllostachys bambusoides  SIEB. Et Zucc,  Phyllostachys nigra  MUNRO,  Phyllostachys nigra  MUNRO var.  henonis  STAPF and  Phyllostachys pubescens  MAZEL ex H. de LEH.