US2011119791A1PendingUtilityA1

Isolated polypeptides, polynucleotides useful for modifying water user efficiency, fertilizer use efficiency, biotic/abiotic stress tolerance, yield and biomass in plants

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Assignee: EVOGENE LTDPriority: Dec 27, 2007Filed: Dec 23, 2008Published: May 19, 2011
Est. expiryDec 27, 2027(~1.5 yrs left)· nominal 20-yr term from priority
Inventors:Gil RonenBasia Judith VinocurAlex DiberSharon AyalHagai KarchiYoav Herschkovitz
C07K 14/415C12N 15/8273C12N 15/8261C12N 15/8271Y02A40/146C12N 15/8242C12N 15/8247C12N 15/8245
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Claims

Abstract

Polynucleotides, polypeptides, plant cells expressing same and methods of using same for increasing abiotic stress tolerance water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant. The method is effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

Claims

exact text as granted — not AI-modified
1 . A method of increasing abiotic stress tolerance, water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 2767, 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2766, 2768-2769, 3052-3065 and 3067-3259, thereby increasing the abiotic stress tolerance, water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of the plant. 
     
     
         2 . A method of increasing abiotic stress tolerance, water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 2756, 7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2755, 2757-2764, 2843-2857 and 2859-3051, thereby increasing the abiotic stress tolerance, water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant. 
     
     
         3 . An isolated polynucleotide comprising a nucleic acid sequence at least 80% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 2756, 7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2755, 2757-2764, 2843-2857 and 2859-3051. 
     
     
         4 . A nucleic acid construct, comprising the isolated polynucleotide of  claim 3  and a promoter for directing transcription of said nucleic acid sequence. 
     
     
         5 . An isolated polypeptide, comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:2767 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2766, 2768-2769, 3052-3065 and 3067-3259. 
     
     
         6 . A plant cell exogenously expressing the isolated polypeptide of  claim 5 . 
     
     
         7 . A plant cell exogenously expressing the isolated polynucleotide of  claim 3 . 
     
     
         8 . The method of  claim 2 , wherein said polynucleotide is selected from the group consisting of SEQ ID NOs:2756 7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2755, 2757-2764, 2843-2857 and 2859-3051. 
     
     
         9 . The method of  claim 1 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:2767 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2766, 2768-2769, 3052-3065 and 3067-3259. 
     
     
         10 . The method of  claim 1 , wherein said polypeptide is selected from the group consisting of SEQ ID NOs: 2767, 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2766, 2768-2769, 3052-3065 and 3067-3259. 
     
     
         11 - 12 . (canceled) 
     
     
         13 . The nucleic acid construct of  claim 4 , wherein said isolated polynucleotide comprising the nucleic acid sequence set forth by SEQ ID NO:2756 7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2755, 2757-2764, 2843-2857, 2859-3050 or 3051. 
     
     
         14 . (canceled) 
     
     
         15 . The isolated polypeptide of  claim 5 , wherein said amino acid sequence is set forth by SEQ ID NO: 2767, 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2766, 2768-2769, 3052-3065, 3067-3258 or 3259. 
     
     
         16 . The plant cell of  claim 6 , wherein said polypeptide having the amino acid sequence set forth by SEQ ID NO: 2767, 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2766, 2768-2769, 3052-3065, 3067-3258 or 3259. 
     
     
         17 . The plant cell of  claim 7 , wherein said polynucleotide comprising the nucleic acid sequence set forth by SEQ ID NO: 2756, 7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2755, 2757-2764, 2843-2857, 2859-3050 or 3051. 
     
     
         18 . The method of  claim 1 , wherein the abiotic stress is selected from the group consisting of salinity, water deprivation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation. 
     
     
         19 . The method of  claim 1 , further comprising growing the plant expressing said exogenous polynucleotide under the abiotic stress. 
     
     
         20 . The nucleic acid construct of  claim 4 , wherein said promoter is a constitutive promoter. 
     
     
         21 . The plant cell of  claim 6 , wherein said plant cell forms a part of a plant. 
     
     
         22 . The plant cell of  claim 7 , wherein said plant cell forms a part of a plant. 
     
     
         23 . The isolated polynucleotide of  claim 3 , wherein said polynucleotide is selected from the group consisting of SEQ ID NOs:2756, 7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2755, 2757-2764, 2843-2857 and 2859-3051. 
     
     
         24 . The method of  claim 1 , wherein the plant is a dicotyledonous plant. 
     
     
         25 . The method of  claim 1 , wherein the plant is a monocotyledonous plant. 
     
     
         26 . The method of  claim 2 , wherein the plant is a dicotyledonous plant. 
     
     
         27 . The method of  claim 2 , wherein the plant is a monocotyledonous plant. 
     
     
         28 . The plant cell of  claim 21 , wherein the plant is a dicotyledonous plant. 
     
     
         29 . The plant cell of  claim 21 , wherein the plant is a monocotyledonous plant. 
     
     
         30 . A transgenic plant exogenously expressing the isolated polynucleotide of  claim 3 . 
     
     
         31 . A transgenic plant transformed with the nucleic acid construct of  claim 4 .

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