Hyperphotosynthetic Organisms

Abstract

The present disclosure identifies pathways and mechanisms to confer improved industrial fitness on engineered organisms. It also discloses engineered organisms having improved industrial fitness. Synthetic biologic engineering modules are disclosed that provide for light capture, carbon dioxide fixation, NADH production, NADPH production, thermotolerance, pH tolerance, flue gas tolerance, salt tolerance, nutrient independence and near infrared absorbance. The disclosed engineered organisms can include one or more of these modules. Also provided are methods of using the engineered organism to produce carbon-based products of interest, biomass or pharmaceutical agents.

1 .- 55 . (canceled) 
     
     
         56 . An engineered cyanobacterial cell for fuel production, wherein said cell comprises a recombinant nucleic acid encoding an enzyme with fructose-1,6-bisphosphatase activity. 
     
     
         57 . The engineered cyanobacterial cell of  claim 56 , wherein said enzyme is  Thermosynechoccocus elongatus  BP-1 fructose-1,6-bisphosphatase. 
     
     
         58 . The engineered cyanobacterial cell of  claim 57 , wherein said cyanobacterial cell is a  Synechococcus  species. 
     
     
         59 . The engineered cyanobacterial cell of  claim 58 , wherein said Synechococcus species is  Synechococcus  sp. PCC 7002. 
     
     
         60 . The engineered cyanobacterial cell of  claim 56 , wherein said cyanobacterial cell is a  Synechococcus  species. 
     
     
         61 . The engineered cyanobacterial cell of  claim 60 , wherein said Synechococcus species is  Synechococcus  sp. PCC 7002. 
     
     
         62 . A method for increasing flux through the Calvin cycle and carbon-fixation in a cyanobacterial cell, comprising transforming said cyanobacterial cell with a nucleic acid encoding an enzyme with fructose-1,6-bisphosphatase activity. 
     
     
         63 . The method of  claim 62 , wherein said enzyme is  Thermosynechoccocus elongatus  BP-1 fructose-1,6-bisphosphatase. 
     
     
         64 . The method of  claim 63 , wherein said cyanobacterial cell is a Synechococcus species. 
     
     
         65 . The method of  claim 64 , wherein said cyanobacterial cell is  Synechococcus  sp. PCC 7002. 
     
     
         66 . A method to produce a carbon-based product of interest, comprising culturing an engineered cyanobacterial cell in the presence of CO2 and light under conditions suitable to produce a carbon-based product of interest, wherein said engineered cyanobacterial cell comprises a recombinant nucleic acid encoding an enzyme with fructose-1,6-bisphosphatase activity. 
     
     
         67 . The method of  claim 66 , wherein said fructose-1,6-bisphosphatase is  Thermosynechoccocus elongatus  BP-1 fructose-1,6-bisphosphatase. 
     
     
         68 . The method of  claim 66 , wherein said cyanobacterial cell is a  Synechococcus  species. 
     
     
         69 . The method of  claim 67 , wherein said cyanobacterial cell is  Synechococcus  sp. PCC 7002. 
     
     
         70 . The method of  claim 68 , wherein said Synechococcus species is  Synechococcus  sp. PCC 7002.