System for ph-neutral stable electrophoresis gel

Abstract

A gel and buffer system for gel electrophoresis wherein separation occurs at neutral pH.

1 - 22 . (canceled) 
     
     
         23 . An apparatus for use in a discontinuous buffer gel electrophoresis system, the apparatus comprising: a slab of precast electrophoresis gel adapted for insertion in an electrophoresis container, the gel uniformly saturated with a gel buffer comprising a primary organic amine or substituted amine with a pK a  near neutrality and titrated with an acid, to a pH between pH 5.5 and pH 8. 
     
     
         24 . The apparatus according to  claim 23 , wherein the pH of the electrophoresis gel is between pH 6 and pH 7.5. 
     
     
         25 . The apparatus according to  claim 23 , wherein the pK a  of the primary organic amine or substituted amine is between pH 6 and pH 8. 
     
     
         26 . The apparatus according to  claim 25 , wherein the pK a  of the primary organic amine or substituted amine is less than 8. 
     
     
         27 . The apparatus according to  claim 23 , wherein the acid is an organic acid. 
     
     
         28 . The apparatus according to  claim 23 , wherein the slab or precast electrophoresis gel is provided in a disposable cassette. 
     
     
         29 . A disposable cassette adapted for insertion in an electrophoresis apparatus, said disposable cassette comprising a precast electrophoresis gel uniformly saturated with a primary organic amine or a substituted amine having a pK a  near neutrality and titrated with an acid to a pH between pH 5.5 and pH 8. 
     
     
         30 . The disposable cassette according to  claim 29 , wherein the pH of the electrophoresis gel is between pH 5.5 and pH 8. 
     
     
         31 . The disposable cassette according to  claim 30 , wherein the pH of the electrophoresis gel is between pH 6 and pH 7.5. 
     
     
         32 . The disposable cassette according to  claim 29 , wherein the pK a  of the primary organic amine or substituted amine is between pH 6 and pH 8. 
     
     
         33 . The apparatus according to  claim 32 , wherein the pK a  of the primary organic amine or substituted amine is less than 8. 
     
     
         34 . The apparatus according to  claim 29 , wherein the acid is an organic acid.