Methods and reagents for vaccination which generate a cd8 t cell immune response

Abstract

New methods and reagents for vaccination are described which generate a CD8 T cell immune response against malarial and other antigens such as viral and tumour antigens. Novel vaccination regimes are described which employ a priming composition and a boosting composition, the boosting composition comprising a non-replicating or replication-impaired pox virus vector carrying at least one CD8 T cell epitope which is also present in the priming composition.

1 - 9 . (canceled) 
     
     
         10 . A method for generating a CD8+ T cell immune response in a mammal against at least one target antigen, comprising administering to the mammal at least one dose of each of the following:
 (i) a priming composition comprising a source of one or more CD8+ T cell epitopes of the target antigen; and   (ii) a boosting composition comprising a source of one or more CD8+ T cell epitopes of the target antigen, including at least one CD8+ T cell epitope which is the same as a CD8+ T cell epitope of the priming composition, wherein the source of the CD8+ T cell epitopes is a non-replicating or replication-impaired recombinant adenovirus vector;   wherein if the source of epitopes in (i) is a viral vector, the viral vector in (ii) is derived from a different virus;   whereby a CD8+ T cell immune response is generated in the mammal.   
     
     
         11 . The method of  claim 10 , further comprising administering an adjuvant.