Extract of toona sinensis from supercritical fluid extraction for treating diabetes and metabolic diseases, the preparation method and the use thereof

Abstract

The Toona sinensis extract of the present invention is prepared using supercritical fluid technique, wherein the method includes steps of: (a) drying the leaves of T. sinensis ; (b) pulverizing the leaves as particles; and (c) extracting the particles with supercritical carbon dioxide to obtain the T. sinensis extract. This supercritical T. sinensis extract not only can decrease blood sugar level, but also promotes lipid degradation, inhibits the formation of huge lipid droplet and improves the metabolic symptoms. Accordingly, the T. sinensis extract further is able to be prepared as food supplement and pharmaceuticals.

1 . A preparation method of a  Tonna sinensis  extract for reducing a blood glucose level, comprising steps of:
 drying a leaf of a  Tonna sinensis;      pulverizing the leaf as particles; and   extracting the particles with a supercritical carbon dioxide to obtain the  T. sinensis  extract.   
     
     
         2 . The method according to  claim 1 , wherein the leaf is one selected from a group consisting of a young leaf, a matured leaf, a young shoot and a combination thereof. 
     
     
         3 . The method according to  claim 1 , wherein the leaf has an aqueous capacity less than 30% after the leaf is dried. 
     
     
         4 . The method according to  claim 1 , wherein the particles have a size less than 5 μm in diameter. 
     
     
         5 . The method according to  claim 1 , wherein the extracting step is performed at a pressure ranged between 350 bar and 550 bar and a temperature between 40° C. and 60° C. 
     
     
         6 . A  Toona sinensis  extract for reducing a blood glucose level comprising at least one of a monoterpene, a monoterpene derivative, a diterpene derivative, a triterpene, a sesquiterpene, sequiterpene derivative, a saturated fatty acid, a unsaturated fatty acid, an alkane, an alkene and a phytosterol. 
     
     
         7 . The extract according to  claim 6 , wherein the monoterpene comprises an α-pinene and an β-pinene, the monoterpene derivative comprises a limonene, the diterpene derivative comprises a phytol, and the triterpene comprises a squalene. 
     
     
         8 . The extract according to  claim 6 , wherein the sesquiterpene comprises an α-cubebene, a copaene, an aromadendrene, a caryophyllene, an α-caryophyllene, a β-caryophyllene, an aristolene, a γ-patchoulene, an α-selinene, a β-selinene, a γ-selinene, an elixnen, a germacrene A, a germacrene B, a germacrene D, a β-elemene, a γ-elemene, a δ-elemene, a τ-elemene, a γ-cadiene, a τ-muurolene, an α-farnesene and a lepidozene, and the sesquiterpene derivative comprises a caryophylene oxide, a spathulenol, a ledene oxide, a germacrene D-4-ol and a globulol. 
     
     
         9 . The extract according to  claim 6 , wherein the saturated fatty acid comprises an ethyl pentadecanoate and an ethyl palmitate, and the unsaturated fatty acid comprises a methyl linoleate, an ethyl 9,12-octadecadienoate and an ethyl α-linolenate. 
     
     
         10 . The extract according to  claim 6 , wherein the alkane has a carbon number not less than 21. 
     
     
         11 . The extract according to  claim 10 , wherein the alkane comprises an n-heneicosane, an n-docosane, an n-tricosane, an n-pentacosane, an n-heptacosane, an n-octacosane, an n-nonacosane, an n-hentriacontane, an n-dotriacontane, an n-pentatriacontane, an n-hexatricontane, an n-tritetracontane and an n-tetratetracontane. 
     
     
         12 . The extract according to  claim 6 , wherein the alkene comprises a (17E)-17-pentatriacontene, and the phytosterol comprises a β-sitosterol. 
     
     
         13 . The extract according to  claim 6 , wherein the extract further comprises a 6-methyl-5-hepten-2-one and a phytone. 
     
     
         14 . The extract according to  claim 6  being prepared by extracting dried particles of a leaf of a  Toona sinensis  with a supercritical carbon dioxide. 
     
     
         15 . The extract according to  claim 14 , wherein the leaf comprises one being selected from a group consisting of a young leaf, a matured leaf, a young shoot and a combination thereof. 
     
     
         16 . The extract according to  claim 6  being manufactured as at least one product of a food and a drug. 
     
     
         17 . The extract according to  claim 6  further being used for improving metabolic diseases and inhibiting an accumulation of fats. 
     
     
         18 . A preparation method of a  Toona sinensis  extract for reducing a blood glucose level, comprising a step of extracting a  Toona sinensis  with a supercritical fluid to obtain the  T. sinensis  extract. 
     
     
         19 . The method according to  claim 18 , wherein the supercritical fluid comprises a carbon dioxide, the  T. sinensis  extract comprises a  Toona sinensis  essential oil, and the  T. sinensis  has a leaf to be prepared as particles. 
     
     
         20 . The method according to  claim 19 , wherein the particles are obtained by sequentially drying and pulverizing the leaf. 
     
     
         21 . A method for detecting at least one component in a  Toona sinensis  extract, comprising a step of determining an amount of the at least one component being one selected from a group consisting of a monoterpene, a monoterpene derivative, a diterpene derivative, a triterpene, a sesquiterpene, sequiterpene derivative, a saturated fatty acid, a unsaturated fatty acid, an alkane, an alkene, a phytosterol, 6-methyl-5-hepten-2-one and a phytone using GC-MS.