US2011171717A1PendingUtilityA1

Method for enhancing polymerase activity

Assignee: KOBAYASHI EIJIPriority: Oct 2, 2006Filed: Nov 26, 2010Published: Jul 14, 2011

Est. expiryOct 2, 2026(~0.2 yrs left)· nominal 20-yr term from priority

Inventors:Eiji Kobayashi Yuki Ueda Yoshimi Sato Takashi Uemori Hiroyuki Mukai Ikunoshin Kato

C12N 9/1241

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Claims

Abstract

A polymerase activity is effectively enhanced by adding an anionic surfactant, in particular an anionic surfactant having a polyethoxyl group, to a reaction mixture containing a polymerase.

Claims

exact text as granted — not AI-modified

1 . A method for enhancing a polymerase activity, the method comprising adding an anionic surfactant having a polyethoxyl group to a reaction mixture containing a polymerase. 
     
     
         2 . The method according to  claim 1 , wherein the anionic surfactant has a sulfonate group or a carboxylate group. 
     
     
         3 . The method according to  claim 1 , wherein the anionic surfactant is selected from the group consisting of allyl alcohol 1,2-butoxylate-block-ethoxylate ammonium sulfate, glycolic acid ethoxylate 4-nonylphenyl ether, glycolic acid ethoxylate oleyl ether and poly(ethylene glycol)4-nonylphenyl 3-sulfopropyl ether as well as salts thereof.

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