Compositions containing platelet contents

Abstract

This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided.

1 - 95 . (canceled) 
     
     
         96 . A platelet lysate composition comprising a filtrate from a lysed platelet preparation passed through a 0.45 μm or smaller filter. 
     
     
         97 . The platelet lysate composition of  claim 96 , wherein said lysed platelet preparation is a lysed apheresis platelet preparation. 
     
     
         98 . The platelet lysate composition of  claim 96 , wherein said platelet lysate composition comprises greater than 200 pg of VEGF polypeptide per mL. 
     
     
         99 . The platelet lysate composition of  claim 96 , wherein culturing 1.4×10 6  mesenchymal stem cells with media containing about five percent of said platelet lysate composition results in greater than 1.4×10 7  cells after three days. 
     
     
         100 . A composition comprising medium and a platelet plasma culture supplement. 
     
     
         101 . The composition of  claim 100 , wherein said platelet plasma culture supplement is a platelet lysate comprising plasma. 
     
     
         102 . The composition of  claim 100 , wherein said platelet plasma culture supplement is a filtrate obtained from filtering a platelet apheresis preparation comprising plasma. 
     
     
         103 . The composition of  claim 102 , wherein said platelet apheresis preparation is a platelet apheresis preparation collected from a human over ten days earlier. 
     
     
         104 . An isolated cell culture comprising cells cultured in medium comprising a platelet plasma culture supplement, wherein said cells grow in said medium at a rate that is faster than the rate of control cells cultured in control medium comprising 10 percent fetal bovine serum. 
     
     
         105 . The isolated cell culture of  claim 104 , wherein said cells and said control cells are primary tumor cells. 
     
     
         106 . The isolated cell culture of  claim 105 , wherein said cells and said control cells are human primary glioma cells. 
     
     
         107 . The isolated cell culture of  claim 104 , wherein 1×10 5  of said cells grow in said medium to more than 3×10 7  of said cells faster than 1×10 5  of said control cells cultured in said control medium grow to 3×10 7  of said control cells. 
     
     
         108 . An isolated cell culture comprising tumor cells or stem cells cultured in medium comprising a platelet plasma culture supplement, wherein said tumor cells grow in said medium in a manner that is more genetically stable than when control tumor cells are cultured in control medium comprising 10 percent fetal bovine serum, and wherein said stem cells grow in said medium in a manner that is more genetically stable than when control stem cells are cultured in control medium comprising 10 percent fetal bovine serum. 
     
     
         109 . The isolated cell culture of  claim 108 , wherein said isolated cell culture comprises said tumor cells, and wherein said tumor cells and said control tumor cells are primary tumor cells. 
     
     
         110 . The isolated cell culture of  claim 109 , wherein said primary tumor cells are human primary glioma cells. 
     
     
         111 . The isolated cell culture of  claim 108 , wherein said isolated cell culture comprises said stem cells, and wherein said stem cells and said control stem cells are mesenchymal stem cells. 
     
     
         112 . The isolated cell culture of  claim 111 , wherein said mesenchymal stem cells are adipose-derived, mesenchymal stem cells. 
     
     
         113 . A method for obtaining a platelet plasma culture supplement comprising removing platelets or platelet debris from a platelet apheresis preparation such that at least some of the plasma within said platelet apheresis preparation is retained, thereby obtaining said platelet plasma culture supplement, wherein:
 (a) said platelet plasma culture supplement comprises the ability to induce proliferation of less than 1×10 6  adipose-derived, mesenchymal stem cells at passage five into at least 1×10 8  cells within 20 days when added to minimal essential medium at a concentration of 5 percent, or (b) minimal essential medium having 5 percent of said platelet plasma culture supplement is capable of establishing, from newly diagnosed human glioblastoma multiforme biopsy tissue, human glioma cultures having greater than 3×10 7  cells within 60 days at 37° C. with a success rate that is greater than 30 percent.   
     
     
         114 . The method of  claim 113 , wherein said platelet plasma culture supplement comprises the ability to induce proliferation of less than 1×10 6  adipose-derived, mesenchymal stem cells at passage five into at least 1×10 8  cells within 15 days when added to minimal essential medium at a concentration of 5 percent. 
     
     
         115 . The method of  claim 113 , wherein minimal essential medium having 5 percent of said platelet plasma culture supplement is capable of establishing, from newly diagnosed human glioblastoma multiforme biopsy tissue, human glioma cultures having greater than 3×10 7  cells within 60 days at 37° C. with a success rate that is greater than 30 percent, and wherein said success rate is greater than 40 percent.