Material for treatment of cerebral infarction and brain tissue regeneration method

Abstract

Disclosed is a therapeutic material for cerebral infarction, which can recover a vascular disorder in an area affected by cerebral infarction to improve the brain function. The therapeutic material for cerebral infarction includes a dental pulp stem cell comprising at least one member selected from a CD105-positive cell, an SP cell, a CD24-positive cell, a CD133-positive cell, a CD271-positive cell and a CD150-positive cell. The therapeutic material may additionally include a protein secreted from a dental pulp cell. A transplanted dental pulp stem cell cannot be differentiated directly into a neural progenitor cell or a neurocyte, but is involved indirectly in the promotion of differentiation to eliminate an area affected by cerebral infarction, thereby recovering the affected area into a normal area.

1 . A material for treatment of cerebral infarction comprising at least any one of: a dental pulp stem cell comprising at least one of a CD105-positive cell, a CD24-positive cell, a CD271-positive cell, and a CD150-positive cell; and a secretory protein of the dental pulp stem cell. 
     
     
         2 . A material for treatment of cerebral infarction comprising at least one of: a dental pulp stem cell comprising at least one of a CD105-positive SP cell, a CD24-positive SP cell, a CD271-positive SP cell, and a CD150-positive SP cell; and a secretory protein of the dental pulp stem cell. 
     
     
         3 . The material for treatment of cerebral infarction according to  claim 1 , wherein the dental pulp stem cell expresses a factor comprising at least one of a cell migration factor, a cell growth factor, an angiogenic factor, and a neurotrophic factor in a peri-infarct region of the brain. 
     
     
         4 . The material for treatment of cerebral infarction according to  claim 3 , wherein the cell migration factor is at least one of SDF1, GCSF, MMP3, Slit, and GMCSF. 
     
     
         5 . The material for treatment of cerebral infarction according to  claim 3 , wherein the neurotrophic factor is at least one of VEGF, NGF, GDNF, BDNF, LIF, MYC, Neurotrophine 3, TP53, and BAX. 
     
     
         6 . The material for treatment of cerebral infarction according to  claim 3 , wherein the cell growth factor is at least one of bFGF and PDGF. 
     
     
         7 . The material for treatment of cerebral infarction according to  claim 3 , wherein the angiogenic factor is at least one of PGF, CXCL1, CXCL2, CXCL3, CXCL5, CXCL10, ANPEP, NRP1, TGFβ, ECGF1, ID1, and CSF3. 
     
     
         8 . The material for treatment of cerebral infarction according to  claim 1 , wherein the dental pulp stem cell has a concentration of 1×10 5  cells/μl to 1×10 7  cells/μl. 
     
     
         9 . The material for treatment of cerebral infarction according to  claim 1 , wherein the dental pulp stem cell is a dental pulp stem cell derived from a permanent tooth or a deciduous tooth. 
     
     
         10 . A brain tissue regeneration method comprising injecting at least one of: a dental pulp stem cell comprising at least one of a CD105-positive cell, a CD24-positive cell, a CD271-positive cell, and a CD150-positive cell; and a secretory protein of the dental pulp stem cell, into the brain striatum after cerebral infarction, thereby regenerating the central nervous tissue of the brain to recover brain function. 
     
     
         11 . (canceled) 
     
     
         12 . The brain tissue regeneration method according to  claim 10 , wherein the dental pulp stem cell expresses a factor comprising at least one of a cell migration factor, a cell growth factor, an angiogenic factor, and a neurotrophic factor in a peri-infarct region of the brain. 
     
     
         13 . The brain tissue regeneration method according to  claim 10 , wherein the dental pulp stem cell has a concentration of 1×10 5  cells/μl to 1×10 7  cells/μl. 
     
     
         14 . The brain tissue regeneration method according to  claim 10 , wherein the dental pulp stem cell is a dental pulp stem cell derived from a permanent tooth or a deciduous tooth.