Use of overground part of hedychium coronarium koenig in reducing blood glucose; extracts and compositions of overground part of hedychium coronarium koenig and their uses

Abstract

The present invention is related to the use of an overground part of Hedychium coronarium Koenig in lowering blood glucose, increasing insulin levels and treating and/or preventing diabetes without overly reducing blood glucose in a subject; i.e., not reducing blood glucose in a fasting subject. The present invention also relates to an extract and composition of the overground part of Hedychium coronarium Koenig and its use in lowering blood glucose, increasing insulin levels and treating and/or preventing diabetes.

1 . A method for lowering blood glucose, increasing insulin levels, reducing insulin resistance, and/or treating and/or preventing diabetes comprising administering an effective amount of an overground part of  Hedychium coronarium  Koenig to a subject. 
     
     
         2 . The method of  claim 1 , wherein the lowering of blood glucose does not occur in a fasting subject. 
     
     
         3 . The method of  claim 1 , wherein the diabetes is type I or type II diabetes. 
     
     
         4 . The method of  claim 1 , wherein the overground part of  Hedychium coronarium  Koenig is the leaves and/or pseudostem of the plant. 
     
     
         5 . The method of  claim 4 , wherein the overground part of  Hedychium coronarium  Koenig is the leaves and pseudostem of the plant. 
     
     
         6 . A method for making an extract of an overground part of  Hedychium coronarium  comprising extracting the overground part of  Hedychium coronarium  Koenig with a solvent. 
     
     
         7 . The method of  claim 6 , wherein the overground part of  Hedychium coronarium  Koenig is the leaves and/or pseudostem of the plant. 
     
     
         8 . The method of  claim 7 , wherein the overground part of  Hedychium coronarium  Koenig is the leaves and pseudostem of the plant. 
     
     
         9 . The method of  claim 6 , wherein the overground part of  Hedychium coronarium  Koenig is extracted with the solvent at a ratio of about 1:1 to about 1:100 (w/v (g/ml)). 
     
     
         10 . The method of  claim 9 , wherein the solvent is selected from the group consisting of water, ethanol, ethanol/water mixture, methanol, butanol, iso-butanol, acetone, hexane, petroleum ether, and ethyl acetate. 
     
     
         11 . The method of  claim 10 , wherein the solvent is selected from the group consisting of water, ethanol and an ethanol/water mixture. 
     
     
         12 . The method of  claim 11 , wherein the water extract is prepared by washing the overground part of  Hedychium coronarium , grinding and drying (at about 60° C.) the plant sample to obtain a ground-dried plant sample, extracting the ground-dried plant sample with water at about 100° C. at a ratio of about 1:10 (w/v (g/ml)) for about 1 hour to obtain a crude product, filtrating and centrifuging the crude product to obtain a water extract, optionally re-extracting the remaining plant sample with water at about 100° C. at a ratio of about 1:10 (w/v (g/ml)) for about 1 hour to obtain a further crude product, filtrating and centrifuging the further crude product to obtain a further water extract, optionally combining the water extracts obtained from the previous two extractions to obtain a combined water extract, and concentrating the combined water extract to obtain a concentrated-combined water extract, and optionally preparing the concentrated-combined water extract at different dosages. 
     
     
         13 . The method of  claim 11 , wherein the ethanol extract is prepared by washing the overground part of  Hedychium coronarium , grinding and drying (at about 60° C.) the plant sample to obtain a ground-dried plant sample, extracting the ground-dried plant sample with 95% ethanol at a ratio of about 1:10 (w/v (g/ml)) for about 16 hours to obtain a crude product, filtrating and centrifuging the crude product to obtain an ethanol extract, optionally re-extracting the remaining plant sample with ethanol at a ratio of about 1:10 (w/v (g/ml)) to obtain a further crude product, filtrating and centrifuging the further crude product to obtain a further ethanol extract, optionally combining the ethanol extracts obtained from the previous two extractions to obtain a combined ethanol extract, and concentrating the combined ethanol extract under reduced pressure at a temperature of about 40° C. to obtain a concentrated-combined ethanol extract, and optionally preparing the concentrated-combined ethanol extract at different dosages. 
     
     
         14 . The method of  claim 11 , wherein the water-ethanol extract is prepared by washing the overground part of  Hedychium coronarium , grinding and drying (at about 60° C.) the plant sample a ground-dried plant sample, extracting the ground-dried plant sample with about 100° C. water at a ratio of about 1:10 (w/v (g/ml)) for about 1 hour to obtain a crude product, filtrating and centrifuging the crude product to obtain a water extract, optionally re-extracting the remaining plant sample with water at about 100° C. at a ratio of about 1:10 (w/v (g/ml)) for about 1 hour to obtain a further crude product, filtrating and centrifuging the further crude product to obtain a further water extract, optionally combining the water extracts obtained from the previous two extractions to obtain a combined water extract, diluting the combined water extract so that the v/w (ml/g) ratio of the diluted water extract to the ground-dried plant sample is about 1:1, adding ethanol to the diluted water extract so that the concentration of ethanol becomes about 70%, standing the ethanol solution for about 20 hours and then centrifuging and concentrating the resulting solution layer. 
     
     
         15 . An extract of an overground part of  Hedychium coronarium  prepared by the method of  claim 6 . 
     
     
         16 . An extract of an overground part of  Hedychium coronarium  prepared by the method of  claim 11 . 
     
     
         17 . An extract of an overground part of  Hedychium coronarium  prepared by the method of  claim 12 . 
     
     
         18 . An extract of an overground part of  Hedychium coronarium  prepared by the method of  claim 13 . 
     
     
         19 . An extract of an overground part of  Hedychium coronarium  prepared by the method of  claim 14 . 
     
     
         20 . A pharmaceutical composition comprising an overground part of  Hedychium coronarium  and/or its extract mixed with suitable pharmaceutically acceptable excipients.