Labelled silica nanoparticles for immunochromatographic reagent, immunochromatographic reagent, immunochromatographic test strip using the same, and immunochromatographic fluorescence-detecting system or radiation-detecting system

Abstract

Labelled silica nanoparticles for immunochromatographic reagent, comprising silica nanoparticles containing a labelled substance.

1 . An immunochromatographic test strip, comprising
 a sample application member,   a member impregnated with an immunochromatographic reagent comprising labelled silica nanoparticles which are surface-modified with an analyte-recognizing substance,   a membrane having an antibody-immobilized region, and   an absorption pad that are connected in series.   
     
     
         2 . The immunochromatographic test strip according to  claim 1 , wherein two or more kinds of analytes are detected simultaneously, based on the difference in hue of color formation or fluorescence wavelength in a single antibody-immobilized region. 
     
     
         3 . The immunochromatographic test strip according to  claim 1 , wherein the silica nanoparticles are both surface-modified with an analyte-recognizing substance and bonded with polyethylene glycol. 
     
     
         4 . An immunochromatographic fluorescence-detecting system, comprising
 an immunochromatographic reagent comprising labelled silica nanoparticles which are surface-modified with an analyte-recognizing fluorescent substance,   wherein the reagent allows high-sensitivity detection by detection of fluorescence emitted from the silica nanoparticles containing the fluorescent substance, and   wherein the wavelength of an excitation light source used for detection of the fluorescence emitted from the silica nanoparticles is 200 nm to 600 nm.   
     
     
         5 . The immunochromatographic fluorescence-detecting system according to  claim 4 , further comprising
 a filter eliminating the excitation light and transmitting only the fluorescence.   
     
     
         6 . The immunochromatographic fluorescence-detecting system according to  claim 5 , further comprising
 a photoelectron multiplier or a CCD detector detecting the fluorescence,   wherein the system allows quantitative determination of the analyte by detection of the fluorescence and measurement of the fluorescent intensity with the photoelectron multiplier or the CCD detector.   
     
     
         7 . The immunochromatographic fluorescence-detecting system according to  claim 4 , wherein the excitation light source is a mercury lamp, a halogen lamp, or a xenon lamp. 
     
     
         8 . The immunochromatographic fluorescence-detecting system according to  claim 4 , wherein the silica nanoparticles are both surface-modified with an analyte-recognizing substance and bonded with polyethylene glycol. 
     
     
         9 . An immunochromatographic radiation-detecting system, comprising
 an immunochromatographic reagent comprising labelled silica nanoparticles which are surface-modified with an analyte-recognizing substance,   wherein radiation emitted from the silica nanoparticles is measured with a Geiger counter or by photosensitizing an X-ray film.   
     
     
         10 . The immunochromatographic radiation-detecting system according to  claim 9 , wherein the silica nanoparticles are both surface-modified with an analyte-recognizing substance and bonded with polyethylene glycol.