Compositions and Methods to Promote Implantation and Engrafment of Stem Cells

Abstract

Tissue repair in-vivo depends on acute inflammation, but in many clinical situations the other major components of healing such as blood supply, anabolic hormones, growth factors, and stem cells are lacking. This invention includes compositions consisting of an agent which induces an inflammatory healing response combined with an autologous platelet lysate at a specific concentration which may have demonstrated in-vitro abilities to expand autologous tissue repair cells.

1 . A composition for facilitating tissue repair in a patient in need thereof comprising a therapeutically effective amount of an autologous platelet lysate in combination with one or more inflammation inducing agent. 
     
     
         2 . The composition of  claim 1 , wherein the inflammation inducing agent is an agent capable of inducing local cell injury, in the patient in need thereof. 
     
     
         3 . The composition of  claim 2 , wherein the agent for inducing local cell injury is selected from the group consisting of hypertonic dextrose, sodium morrhuate, inflammatory cytokines, pumice, phenol and mixtures thereof. 
     
     
         4 . The composition of  claim 2 , wherein the agent for inducing local cell injury acts through local cell lysis via a hyper or hypo osmolar agent. 
     
     
         5 . The composition of  claim 1 , wherein platelet rich plasma, platelet rich fibrin, or another whole platelet concentrate is substituted for platelet lysate. 
     
     
         6 . The composition of  claim 1 , wherein cytokines are added to the 5% to 40% platelet lysate to alter the timing of growth factor degranulation off of platelets in the platelet lysate. 
     
     
         7 . The composition of  claim 1 , further comprising one or more essential nutrients wherein the essential nutrients further facilitate tissue repair. 
     
     
         8 . The composition of  claim 7 , wherein the one or more essential nutrient is selected from the group consisting of glycoaminosglycan's, collagen, amino acids, peptides, proteins, sodium pyruvate, glucose, glutamine, ribonucleosides, deoxyribonucleosides, carbohydrates, and essential oils. 
     
     
         9 . The composition of  claim 1 , further comprising one or more anabolic hormones added to amplify local healing effects. 
     
     
         10 . The composition of  claim 1 , wherein the inflammation inducing agent further comprises a gel, hydrogel, foam, or other material to localize or maximize the micro-injury to an area of application. 
     
     
         11 . A method for facilitated tissue repair in a subject in need thereof comprising:
 obtaining an amount of platelets from the subject;   preparing a 5% to 40% platelet lysate solution from the platelets;   administering an inflammation inducing agent to the subject in an amount sufficient to induce local inflammation at the site in need of tissue repair; and   administering the 5% to 40% platelet lysate solution to the subject at the site in need of tissue repair;   wherein the combined administration of the inflammation inducing agent and platelet lysate facilitates tissue repair at the site in the subject in need of tissue repair.   
     
     
         12 . The method of  claim 11 , further comprising determining the % platelet lysate for administering to the patient by first determining what % of platelet lysate is necessary to optimally culture expand the patient's mesenchymal stem cells in vitro and using this same % platelet lysate for administering to the patient. 
     
     
         13 . The method of  claim 11 , where administering to the patient is to the site in need of repair through a surgical incision, arthroscopically or percutaneously. 
     
     
         14 . The method of  claim 11 , wherein the % platelet lysate and the amount administered, is determined based on at least the patient's age, health, repair site, and level of repair required. 
     
     
         15 . A composition for facilitating tissue repair in a patient in need thereof comprising a therapeutically effective amount of one or more recombinant growth factors in combination with one or more inflammation inducing agent(s). 
     
     
         16 . The composition of  claim 15 , wherein the inflammation inducing agent is selected from the group consisting of hypertoxic dextrose, sodium morrhuate, inflammatory cytokines, phenol and mixtures thereof. 
     
     
         17 . The composition of  claim 15 , further comprising 5% to 40% platelet lysate wherein the platelet lysate is prepared from whole blood harvested from the patient in need of tissue repair.