Yersinia SPP. Polypeptides and Methods of Use

Abstract

The present invention provides isolated polypeptides isolatable from a Yersinia spp. Also provided by the present invention are compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.

1 . A composition comprising:
 two isolated polypeptides having molecular weights of 83 kDa, 70 kDa, 66 kDa, or a combination thereof, and two isolated polypeptides having molecular weights of 40 kDa, 38 kDa, or 37 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides having a molecular weight of 83 kDa, 70 kDa, or 66 kDa are isolatable from a  Yersinia enterocolitica  when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator, and wherein the composition protects a mouse against challenge with  Y. enterocolitica  ATCC strain 27729.   
     
     
         2 - 62 . (canceled) 
     
     
         63 . A composition comprising a polypeptide comprising the amino acid sequence of SEQ ID No 132. 
     
     
         64 . The composition of  claim 63  wherein the polypeptide is covalently bound to a carrier polypeptide. 
     
     
         65 . The composition of  claim 63  further comprising a pharmaceutically acceptable carrier. 
     
     
         66 . The composition of  claim 63  further comprising amino acids 5-10 of SEQ ID NO:119. 
     
     
         67 . The composition of  claim 66  wherein the polypeptide is covalently bound to a carrier polypeptide. 
     
     
         68 . The composition of  claim 66  further comprising a pharmaceutically acceptable carrier. 
     
     
         69 . A method comprising:
 administering to a subject a composition in an amount effective to treat an infection caused by a gram negative microbe, wherein the composition comprises:   a polypeptide comprising the amino acid sequence of SEQ ID No 132; or   antibody that specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID No 132.   
     
     
         70 . A method comprising:
 administering to a subject a composition in an amount effective to decrease colonization of the subject by a gram negative microbe, wherein the composition comprises:   a polypeptide comprising the amino acid sequence of SEQ ID No 132; or   antibody that specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID No 132.   
     
     
         71 . A method comprising:
 administering to a subject a composition in an amount effective to treat a symptom of a condition caused by infection by a gram negative microbe, wherein the composition comprises:   a polypeptide comprising the amino acid sequence of SEQ ID No 132; or   antibody that specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID No 132.   
     
     
         72 . A composition comprising a polypeptide comprising the amino acid sequence of SEQ ID No 42. 
     
     
         73 . The composition of  claim 72  wherein the polypeptide is covalently bound to a carrier polypeptide. 
     
     
         74 . The composition of  claim 72  further comprising a pharmaceutically acceptable carrier. 
     
     
         75 . The composition of  claim 74  further comprising amino acids 10-20 of SEQ ID NO:56. 
     
     
         76 . The composition of  claim 74  further comprising amino acids 17-22 of SEQ ID NO:57. 
     
     
         77 . The composition of  claim 76  further comprising amino acids 10-20 of SEQ ID NO:56. 
     
     
         78 . A method comprising:
 administering to a subject a composition in an amount effective to treat an infection caused by a gram negative microbe, wherein the composition comprises:   a polypeptide comprising the amino acid sequence of SEQ ID No 42; or   antibody that specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID No 42.   
     
     
         79 . A method comprising:
 administering to a subject a composition in an amount effective to decrease colonization of the subject by a gram negative microbe, wherein the composition comprises:   a polypeptide comprising the amino acid sequence of SEQ ID No 42; or   antibody that specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID No 42.   
     
     
         80 . A method comprising:
 administering to a subject a composition in an amount effective to treat a symptom of a condition caused by infection by a gram negative microbe, wherein the composition comprises:   a polypeptide comprising the amino acid sequence of SEQ ID No 42; or   antibody that specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID No 42.   
     
     
         81 . A composition comprising amino acids 1-9 of SEQ ID NO:327, amino acids 10-18 of SEQ ID NO:344, and amino acids 1-7 of SEQ ID NO:326. 
     
     
         82 . The composition of  claim 81  further comprising amino acids 5-10 of SEQ ID NO:329.